A well thought out visual and lighshow - coupled with lasers, streamand strobes created a real spectasurely helping to win over anydragged along with a mere half intein
In another strategy, Cas9 is splitted and packaged into parallel AAV particles; each Cas9 part is fused to a split intein
which fuses the Cas9 parts together upon coexpression .
(intervening proteins) are proteins that perform protein-splicing posttranslational modifications  with the goal of creating a regulated Cas9; SpCas9 has been recombined with the intein
37R3-2 [69, 70].
(39) Segmental labeling by either intein
splicing reaction or chemical ligation (40,41) was reported.
The researchers achieved this by exploiting a type of protein known as an 'intein
' - - a short protein that can be inserted into a larger protein, splitting it into two pieces.
Wang, "Recombinant expression of porcine lactoferrin peptide LF-6 with intein
technology and its immunomodulatory function in ETEC K88-infected mice," International Immunopharmacology, vol.
Construction and complementation of a recA deletion mutant of Mycobacterium smegmatis reveals that the intein
in Mycobacterium tuberculosis recA does not affect RecA function.
Xu, "Protein splicing involving the Saccharomyces cerevisiae VMA intein
: the steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system," The Journal of Biological Chemistry, vol.
Pharmavite LLC offers Natare Made Eye Defense, which is formulated with intein
and zeaxanthin--another carotenoid--that help to maintain a healthy macula.
The expressed protein was intein
tagged and purified by binding the tagged protein to a chitin column and then performing an on-column cleavage of the intein
coli as a fusion protein with an intein
tag that contains a ch itin-binding domain.
A small portion of RecA, dubbed the intein
, quickly cuts itself out of the protein and melds the two remaining fragments together to form the final, active molecule.