tb strains that appear to have either one or no copy of IS6110 insertion element
. Thus, PCR diagnosis based exclusively on the IS6110 sequence could lead to a significant number of false negative results.
tuberculosis, the insertion element IS6110 has been studied most intensively (Lonnroth and Raviglione, 2008).
tuberculosis from Asia either do not contain this insertion sequence or contain only one or two copies of the IS6110 insertion element, making the use of this sequence unreliable for the detection and typing of M.
Primers IS1(5'-TGGGTCCGCCAGGCCCAGGTC-3') and IS3 (5'-GTAGGCGTCGGTGACAAAGGC-3') were synthesized using the IS6110 DNA sequence  and used to PCR amplify a 665pb DNA fragment located between bases 211 and 876 of this insertion element of a M.
In this study, we also used the characteristic insertion element IS6110 of M.
Our results agree with previous works [7,27,32] utilizing the detection of insertion element IS6110 in Brazilian M.
Identification of a novel insertion element, IS/548, in group B streptococci, predominantly in strains causing endocarditis.
We also tested whether molecular variation in a region of the chromosome with multiple direct repeat (DR) nucleotide sequences and restriction fragment length polymorphism (RFLP) analysis with insertion elements IS1548 (9) and IS1562 (10) would differentiate M1 isolates.
(15.) DNA insertion elements
, plasmids, and episomes.