There are three types of NOS: neuronal NOS (nNOS), endothelial NOS (eNOS), and inducible NOS
There are three distinct isoforms of NOS in mammalian cells including neuronal NOS (nNOS, Type I), inducible NOS
(iNOS, Type II) and endothelial NOS (eNOS or Type III) (4).
IHC and scoring were conducted as described. Primary antibodies of inducible NOS
(iNOS) (mouse, Abcam, ab129372), neuronal NOS (nNOS) (rabbit, Abcam, ab3511), Cav1.2 (mouse, Abcam, ab84814), Cav1.3 (mouse, Abcam, ab85491), IP[sub]3R1 (rabbit, Abcam, ab125076), IP[sub]3R2 (rabbit, Millipore, ab9074), IP[sub]3R3 (rabbit, Millipore, ab9076), and RyR2 (rabbit, Chemicon, ab9080) were diluted at 1:100.
Three isoforms of NOS have been identified: neuronal NOS (nNOS), inducible NOS
(iNOS) and endothelial NOS (eNOS).
NO is produced from L-arginine by NOS , an enzyme with different isoenzymes (neuronal NOS or nNOS, inducible NOS
or iNOS, endothelial NOS or eNOS, and mitochondrial NOS or mtNOS).
Wu and Meininger (2002) demonstrated that inadequate intake of dietary arginine impairs NO synthesis by both constitutive and inducible NOS
in animals, indicating a role of adequate arginine nutrition in immune function.
The isoforms of NOS are subdivided into three basic categories: endothelial NOS, neuronal NOS and inducible NOS
(iNOS), all of which are encoded by separate genes and, therefore, differently regulated.
Neuronal and inducible NOS
are more commonly found in their soluble form than the membrane-bound one.
NSO and volatile component of NS seed, TQ were found to improve antioxidant capacity induced by several agents in different animal tissues by suppressing oxidative/nitrosative stress, N[O.sup.*] overproduction and inducible NOS
Thus, the dynamic reduction activities ofNO and eNOS occurred against a background of increasing the activity of inducible NOS
and concentration of [ONO-.sub.2] (Table 1).
Oxidative stress is known to upregulate the synthesis of neuronal and inducible NOS
, leading to overproduction of NO, which acts as a neurodestructive agent by the production of peroxynitrites.
NOSs are classified to three forms, that is, endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS