Zhou et al., "The protective action of ketanserin against lipopolysaccharide-induced shock in mice is mediated by inhibiting inducible NO synthase
expression via the MEK/ERK pathway," Free Radical Biology and Medicine, vol.
Stuehr, "Intracellular assembly of inducible NO synthase
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,' Journal of Leukocyte Biology, vol.
These so-called classically activated macrophages, or M1 macrophages, produce large amounts of proinflammatory cytokines, reactive oxygen intermediates, and such reactive nitrogen intermediates as nitric oxide (NO), which is generated by inducible NO synthase
(iNOS) encoded by the Nos2 gene [4, 5].
Inducible NO synthase
: Role in cellular signalling.
This cardioprotective effect was completely abolished with the use of either a NO inhibitor or an inducible NO synthase
blocker, affirming resveratrol's beneficial action.
Muscular structure, enzyme activity, constitutive and inducible nitric oxide (NO) synthases (endothelial NO oxide synthase [eNOS], neuronal NO synthase [nNOS] and inducible NO synthase
[iNOS]), nitrites, nitrates, nitrotyrosine, and the presence of macrophages were analyzed.
A key proinflammatory mediator produced in the intestinal mucosa is the free radical nitric oxide (NO), synthesized by inducible NO synthase
(iNOS).[33,34] Inflammatory stimuli, such as endotoxin and the cytokines IL-1 and TNF-[Alpha], can induce iNOS in a variety of inflammatory cells, including macrophages and neutrophils, and in vascular endothelium and smooth muscle.[35-36] Studies in patients with IBD have shown a marked increase in NO synthesis and iNOS activity in the inflammatory mucosa.[37-38]
Inducible NO synthase
(iNOS) activity stimulated at the site of infection is primarily responsible for the sustained release of NO (2).
To assess nitrosative stress, adjacent brain sections were stained for nitrotyrosine, inducible NO synthase
(iNOS), neuronal NO synthase (nNOS), and endothelial NO synthase (eNOS), respectively.
NO is synthesized from L-arginine (Arg) by constitutive and inducible NO synthase