in situ hybridization

(redirected from In-situ hybridisation)
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in situ hybridization

a technique developed in 1969 for annealing nucleic acid probes to cellular DNA for detection by autoradiography. Under proper laboratory conditions, the binding process occurs spontaneously. In situ hybridization constitutes a key step in DNA fingerprinting.

in situ hybridization

A method for localizing a sequence of DNA, mRNA, or protein in a cell or tissue; the use of a DNA or RNA probe to detect a cDNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells; ISH is used to map gene sequences to chromosomal sites and detect gene expression. See cDNA, Chromosome, CISH–Chromogenic in situ hybridization, DNA sequence, Eukaryote, FISH–Fluorescent in situ hybridization, GISH–Genomic in situ hybridization, Gene expression, Gene mapping, Hybridization, Immunoblotting, Interphase, Probe. Cf Southern blot.

in si·tu hy·brid·i·za·tion

(in sī'tū hī'brid-ī-zā'shŭn)
A technique for annealing nucleic acid probes to cellular DNA for detection by autoradiography; a key step in DNA fingerprinting.

in situ hybridization

HYBRIDIZATION of particular NUCLEIC ACID sequences, for example mRNA, in cells or tissues.
References in periodicals archive ?
Advanced Cell Diagnostics' certified service provider programme is a partnership scheme aimed at ensuring the delivery of the highest-quality RNA in-situ hybridisation data available using the RNAscope assay.
NeoGenomics is a high-complexity CLIA-certified clinical lab that focuses on cancer genetics testing, including cytogenetics, fluorescence in-situ hybridisation (FISH), flow cytometry, immunohistochemistry, morphology studies, anatomic pathology and molecular genetic testing.
The genetic abnormalities are analysed utilising various methods, such as sequencing, Fluorescent In-Situ Hybridisation (FISH), methylation analysis, fragment length analysis and SNP-cytogenetic array technology.
The study compared the accuracy of immunochemistry (IHC) and fluorescent in-situ hybridisation (FISH) data with MammaPrint and BluePrint.