in situ hybridization

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in situ hybridization

a technique developed in 1969 for annealing nucleic acid probes to cellular DNA for detection by autoradiography. Under proper laboratory conditions, the binding process occurs spontaneously. In situ hybridization constitutes a key step in DNA fingerprinting.
Farlex Partner Medical Dictionary © Farlex 2012

in situ hybridization

A method for localizing a sequence of DNA, mRNA, or protein in a cell or tissue; the use of a DNA or RNA probe to detect a cDNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells; ISH is used to map gene sequences to chromosomal sites and detect gene expression. See cDNA, Chromosome, CISH–Chromogenic in situ hybridization, DNA sequence, Eukaryote, FISH–Fluorescent in situ hybridization, GISH–Genomic in situ hybridization, Gene expression, Gene mapping, Hybridization, Immunoblotting, Interphase, Probe. Cf Southern blot.
McGraw-Hill Concise Dictionary of Modern Medicine. © 2002 by The McGraw-Hill Companies, Inc.

in si·tu hy·brid·i·za·tion

(in sī'tū hī'brid-ī-zā'shŭn)
A technique for annealing nucleic acid probes to cellular DNA for detection by autoradiography; a key step in DNA fingerprinting.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

in situ hybridization

HYBRIDIZATION of particular NUCLEIC ACID sequences, for example mRNA, in cells or tissues.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
The patent will include the use of the product in patients with a fluorescence in situ hybridisation of approximately less than 2.0, as a stand-alone therapy or in combination with an adjuvant and/or other agents (e.g.
8 May 2014 - US microarray technology and genomics analysis specialist Affymetrix Inc (NASDAQ:AFFX) and Leica Biosystems, a developer of anatomic pathology workflow solutions, today introduced Affymetrix's fully-automated RNA in situ hybridisation assays, ViewRNA eZ Assays, for use on Leica's BOND RX staining platform.
Identification of monosomy 3 in choroidal melanoma by chromosome in situ hybridisation. Br J Ophthalmol.
IHC and in situ hybridisation (ISH) accounted for 97%, while DNA sequencing identified mutations in 34%.
M2 PHARMA-December 12, 2013-Enzo Biochem, Flagship Biosciences to co-develop companion diagnostics for in situ hybridisation
Fluorescence in situ hybridisation for the detection of bladder cancer.
RNAscope identifies mRNA by in situ hybridisation (ISH), as well as enables detection and quantification of any analyte in formalin-fixed, paraffin-embedded material.
Prediction of prognosis in patients with uveal melanoma using fluorescence in situ hybridisation. Br J Ophthalmol.