immunoprecipitation

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immunoprecipitation

[im″u-no-pre-sip″ĭ-ta´shun]

precipitation resulting from interaction of specific antibody and antigen.

im·mu·no·pre·cip·i·ta·tion

(im'yū-nō-prē-sip'i-tā'shŭn),

The phenomenon of aggregation of sensitized antigen on addition of specific antibody (precipitin) to antigen in solution.

Synonym(s): immune precipitation

Farlex Partner Medical Dictionary © Farlex 2012

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References in periodicals archive ?

To determine if the antibody inhibited [beta]3 phosphorylation, kidney homogenate (200 [micro]g) was immunoprecipitated with the anti-[micro]3 antibody (R2949) using a 1: 500 dilution.

Blocking [alpha]V[beta]3 integrin ligand occupancy inhibits the progression of albuminuria in diabetic rats

To assess the influence of AA polymorphisms at position 156 on the association of HLA-A* 24 variants with TAP, HLA-A*24/156 transduced 721.220 or 721.221 cells were immunoprecipitated with anti-TAP1 ab.

A micropolymorphism altering the residue triad 97/114/156 determines the relative levels of tapasin independence and distinct peptide profiles for HLA-A*24 allotypes

No migration with FBP1 was seen when cell extracts were immunoprecipitated with the control immunoglobulin G (IgG) antibody.

FBP1 is an interacting partner of menin

The immunoprecipitated Ku is not as abundant in Y6 oocytes as in Y5 oocytes.

DNA-dependent protein phosphorylation activity in Xenopus is coupled to a Ku-like protein

Immunoprecipitated single-end sequence data were analyzed using MACS (release 1.4.2, http://liulab.dfci.harvard.edu/MACS/) to find genomic regions that were enriched for specifically precipitated DNA fragments.

Characterization and functional inferences of a genome-wide DNA methylation profile in the loin (longissimus dorsi) muscle of swine

In the latter scenario, when spinophilin is immunoprecipitated, it can extract multiple NF-M molecules, whereas immunoprecipitating multiple NF-M molecules would only extract a few (below the limit of detection) spinophilin molecules.

Mechanisms and Consequences of Dopamine Depletion-Induced Attenuation of the Spinophilin/Neurofilament Medium Interaction

TAK1 was immunoprecipitated from LPS-stimulated untreated cells and from cells treated with torilin, as indicated in Figure 6(d).

Torilin Inhibits Inflammation by Limiting TAK1-Mediated MAP Kinase and NF-[kappa]B Activation

(a) Proteins from each group were extracted and immunoprecipitated with anti-Fas antibody.

Extrinsic Apoptosis Pathway Altered by Glycogen Synthase Kinase-3[beta] Inhibitor Influences the Net Drug Effect on NSC-34 Motor Neuron-Like Cell Survival

SOD2 was immunoprecipitated with anti-Flag antibodies (M2; Sigma, St.

Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

(A-D) Chromatin immunoprecipitated with anti-acetylated H3K9 (H3K9ac; A,C) or anti-E2F1 (B,D) antibodies after exposure of HEK293T cells to 0.5 [micro]M or 2.5 [micro]M [As.sub.2][O.sub.3] for 3 or 72 hr.

E2F1-mediated FOS induction in arsenic trioxide-induced cellular transformation: effects of global H3K9 hypoacetylation and promoter-specific hyperacetylation in vitro

The immunoprecipitated protein was separated by reduced or nonreduced SDS-PAGE, and then transferred to polyvinylidene difluoride membranes.

Construction of a chimeric secretory IgA and its neutralization activity against avian influenza virus H5N1

For the analysis of phospho-VEGFR3 levels, the cell lysates were immunoprecipitated with anti-phospho-tyrosine mouse monoclonal antibody-conjugated sepharose beads (Cell Signaling Technology) overnight, and then subjected to electrophoresis in a 10% SDS-polyacrylamide gel, and used for Western blotting with anti-VEGFR-3 and anti-[beta]-actin antibodies (Sigma).

Anti-tumor and anti-metastatic actions of wogonin isolated from Scutellaria baicalensis roots through anti-lymphangiogenesis