immunoprecipitation

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Related to Immunoprecipitated: Coimmunoprecipitation

immunoprecipitation

 [im″u-no-pre-sip″ĭ-ta´shun]
precipitation resulting from interaction of specific antibody and antigen.

im·mu·no·pre·cip·i·ta·tion

(im'yū-nō-prē-sip'i-tā'shŭn),
The phenomenon of aggregation of sensitized antigen on addition of specific antibody (precipitin) to antigen in solution.
References in periodicals archive ?
To determine if the antibody inhibited [beta]3 phosphorylation, kidney homogenate (200 [micro]g) was immunoprecipitated with the anti-[micro]3 antibody (R2949) using a 1: 500 dilution.
To assess the influence of AA polymorphisms at position 156 on the association of HLA-A* 24 variants with TAP, HLA-A*24/156 transduced 721.220 or 721.221 cells were immunoprecipitated with anti-TAP1 ab.
No migration with FBP1 was seen when cell extracts were immunoprecipitated with the control immunoglobulin G (IgG) antibody.
The immunoprecipitated Ku is not as abundant in Y6 oocytes as in Y5 oocytes.
Immunoprecipitated single-end sequence data were analyzed using MACS (release 1.4.2, http://liulab.dfci.harvard.edu/MACS/) to find genomic regions that were enriched for specifically precipitated DNA fragments.
In the latter scenario, when spinophilin is immunoprecipitated, it can extract multiple NF-M molecules, whereas immunoprecipitating multiple NF-M molecules would only extract a few (below the limit of detection) spinophilin molecules.
TAK1 was immunoprecipitated from LPS-stimulated untreated cells and from cells treated with torilin, as indicated in Figure 6(d).
(A-D) Chromatin immunoprecipitated with anti-acetylated H3K9 (H3K9ac; A,C) or anti-E2F1 (B,D) antibodies after exposure of HEK293T cells to 0.5 [micro]M or 2.5 [micro]M [As.sub.2][O.sub.3] for 3 or 72 hr.
The immunoprecipitated protein was separated by reduced or nonreduced SDS-PAGE, and then transferred to polyvinylidene difluoride membranes.
For the analysis of phospho-VEGFR3 levels, the cell lysates were immunoprecipitated with anti-phospho-tyrosine mouse monoclonal antibody-conjugated sepharose beads (Cell Signaling Technology) overnight, and then subjected to electrophoresis in a 10% SDS-polyacrylamide gel, and used for Western blotting with anti-VEGFR-3 and anti-[beta]-actin antibodies (Sigma).