immunoblotting


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Related to immunoblotting: immunoprecipitation

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', im'yū-nō-blot'ing),
Process by which antigens can be separated by electrophoresis and allowed to adhere to nitrocellulose sheets where they bind nonspecifically and then are subsequently identified by staining with appropriately labeled antibodies.
See also: Western blot analysis.

immunoblotting

[-blot′ing]
a method for identifying antigens. The antigens are allowed to adhere to cellulose sheets and are identified by staining with labeled antibodies. The method is also used to detect monoclonal proteins. See also Western blot test.

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', -ing)
Process by which antigens can be separated by electrophoresis and blotted to nitrocellulose sheets, where they bind and are subsequently identified by staining with labeled antibodies.
See also: Western blot analysis

immunoblotting,

n the immunologic methods for isolating and quantitatively measuring immunoreactive substances. When used with immune reagents such as monoclonal antibodies, the process is known generically as
Western blot analysis.
References in periodicals archive ?
One hundred and seventeen samples from cSLE patients ( n = 46) or children with AS ( n = 11) and HSP ( n = 60) were analyzed using an immunoblotting assay to generate autoantibody profiles.
Immunoprecipitation and subsequent immunoblotting procedures revealed secretion of PCSK9-WT into the medium but detected no PCSK9-Q152H secretion into the medium (Fig.
According to ELISA and immunoblotting studies, these seven MAbs can be categorized into two groups: 1) Four IgG1, 2, 4 specific MAbs of which three (1F5A8, 8F9G7 and 6F11E1), react with conformational epitopes located on heavy chain of IgG1, 2, 4 (Figure 1) and an unusual IgG3 myeloma protein (Goe) bearing allotypic marker of the Mongloid populations [G3m(st)] displaying similar specificity to SPA.
Immunoblotting was the best method of visualizing the 1H12 antigen, since the antigen stained only lightly with a silver stain kit and not at all with Coomassie Blue or Stains-All (data not shown), even after the antigen was concentrated by immunoprecipitation.
Cross-reactivity between the major and hypoallergen components was observed in immunoblotting and crossed-immunoelectrophoresis and fused-rocket immunoelectrophoresis using rabbit antisera to the purified major and hypoallergens, suggesting that the hypoallergen carries a dominant epitope of the major allergen.
com) announces updates to their Immunoblotting database.
To clarify cross-reactivity between royal jelly and honeybee venom, competitive IgE enzyme-linked immunosorbent assay (ELISA) inhibition tests and immunoblotting inhibition assays were performed.
FPLC can be performed both for analytical purposes and as a preparative technique prior to the use of other methods for further characterisation such as 1D and 2D gel electrophoresis, immunoblotting, mass spectrometry and other characterisation methods.
The aim of this study was to investigate the potential synergistic effects of curcumin and resveratrol on IL-1 beta-stimulated human chondrocytes in vitro using immunoblotting and electron microscopy.
ELISA data [micro]g/L) were significantly and positively correlated with the immunoblotting data (U) previously observed following purification with RAP affinity chromatography (r = 0.
Therefore we undertook this study to compare the antibody profile in patients with uncomplicated typhoid fever with those having ileal perforation by immunoblotting.
Furthermore, MOD-5 was engineered to express V5 and HIS epitope tags on the c-terminus for use in immunoblotting and purification studies (MOD-5-VH).