immunoblotting


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Related to immunoblotting: immunoprecipitation

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', im'yū-nō-blot'ing),
Process by which antigens can be separated by electrophoresis and allowed to adhere to nitrocellulose sheets where they bind nonspecifically and then are subsequently identified by staining with appropriately labeled antibodies.
See also: Western blot analysis.

immunoblotting

[-blot′ing]
a method for identifying antigens. The antigens are allowed to adhere to cellulose sheets and are identified by staining with labeled antibodies. The method is also used to detect monoclonal proteins. See also Western blot test.

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', -ing)
Process by which antigens can be separated by electrophoresis and blotted to nitrocellulose sheets, where they bind and are subsequently identified by staining with labeled antibodies.
See also: Western blot analysis

immunoblotting,

n the immunologic methods for isolating and quantitatively measuring immunoreactive substances. When used with immune reagents such as monoclonal antibodies, the process is known generically as
Western blot analysis.
References in periodicals archive ?
1% (w/v) sarkosyl and analyzed by electrophoresis, immunoblotting, and high sensitivity chemiluminescence (27,28), using monoclonal antibody ICSM 35 against PrP to detect ovine PrP.
2: PSemiquantitative immunoblotting of whole kidney protein samples from obstructed (OBS) and non-obstructed kidneys (Non-OBS) from PUUO-9 W and PUUO-15 W rats, and kidneys from matched sham-operated rats (SHAM).
For immunoblotting, 5[micro]L conditioned medium of hEL-myc/CHO 53A5 was used for 12% SDS-PAGE and transferred to nitrocellulose membrane (Bio-Rad).
Purohit (36) reported that immunoblotting showed humoral response against HMD cross-linked allogenic acellular dermal graft.
1A), whereas 5 bands and 3 main bands were found using SDS-PAGE and immunoblotting analysis, respectively (Fig.
The expression of HPV-16 L1 in the selected yeast clone was analyzed by immunoblotting after incubation for 0, 12, 24, 36, 48, 60, 72 h in a shake-flask culture to optimize induction time for optimum expression of recombinant protein.
Immunoblotting was the best method of visualizing the 1H12 antigen, since the antigen stained only lightly with a silver stain kit and not at all with Coomassie Blue or Stains-All (data not shown), even after the antigen was concentrated by immunoprecipitation.
The database currently tags 67,000 individuals working in Immunoblotting.
For immunoblotting, myocytes were plated in laminin-coated polystyrene culture dishes and incubated in a solution of Medium-199 (catalog no.
Contract award: supply of reagents and laboratory tests for the diagnosis of autoantibodies, antibodies against borrelia, matrix metalloproteinase 3, hla-b27 and the immune complexes using enzyme immunoassay methods elisa, indirect immunofluorescence, flow cytometry and immunoblotting for the laboratory department of rheumatology and internal diseases, medical university no.
Here we report on the demonstration of putative human TMPOdelta and epsilon by immunoblotting of human cell lines using a newly prepared polyclonal antiserum against the common N-terminal region of TMPO.
Validation of a western immunoblotting procedure for bovine PrP(Sc) detection and its use as a rapid surveillance method for the diagnosis of bovine spongiform encephalopathy (BSE).