immobilized enzymes

immobilized enzymes

CELLS (alive or dead) or ENZYMES anchored to a solid support for use in BIOCONVERSIONS. A continuous flow of substrate over the immobilized cells or enzymes will enable conversion to the desired product. Immobilized enzymes or dead cells are used for simple one- or two-step bioconversions. However, where multiple steps are involved, living cells are needed. Immobilized systems are used in industry to make various products, such as high-FRUCTOSE syrups, ASPARTIC ACID and semi-synthetic ANTIBIOTICS.
References in periodicals archive ?
Indeed, this result about the optimum pH alteration was expected because of the context of the environments of free and immobilized enzymes as previously indicated.
Furthermore, immobilized enzymes are easily separated from products and used in the continuous production processes in the industry.
The activity of the immobilized enzymes reached a maximum value at about 50[degrees]C, compared to around 40 and 50[degrees]C for the native [beta]-galactosidase and galactose oxidase, respectively, reported previously [57, 58].
The effects of pH and temperature variations on the hydrolytic activity of the immobilized enzymes were examined.
There are some reports showing efforts to unveil the properties and characteristics of new hybrid materials with an organic/inorganic composition seeking future prospects for industrial applications of immobilized enzymes requiring nonharmful or toxic agents [7, 13].
Reusability of Immobilized Enzymes Under MW Irradiation
HPAC and affinity chromatography have also been employed in examining the binding of various solutes and drugs with the transport proteins [[alpha].sub.1]-acid glycoprotein (AGP) or human serum albumin 1HSA) (10, 17, 18), the binding of urediofibrate-like dual agonists with peroxisome proliferator-activated receptors (19), and the interactions of many compounds with immobilized enzymes or lectins (12, 20--22).
Because enzymes are generally sensitive to heat, enhanced thermal stability is among the most important properties of immobilized enzymes for applications.
The response of the biosensors as a function of the relative concentration of the two immobilized enzymes has been investigated and the best working conditions have been identified by measuring the linear range response and sensitivity of each prepared biosensor.
The pH is an important factor that influences the hydrolytic activity of both free and immobilized enzymes, since it can change the performance of the support, the enzyme conformation and the enzymatic reaction.
The RA can be considered more precise immobilization yield than IY since the first is based on the enzyme activity measured in the derivatives (immobilized enzymes).