Batch tests were carried out for 10 min, using the same mass of immobilized enzyme
Recent Advances and Applications of Immobilized Enzyme
Technologies: A Review.
The catalytic activity of the immobilized enzyme
was determined by mixing 1 g of catalytic P (AN-co-MMA) nanoparticles with 50 mL of 100 mM lactose in phosphate-citrate solution (pH 4.
Magnetic beads  have been used to immobilize yeast invertase, and the separation experiment of immobilized enzyme
from substrate was easy to operate.
This process was the first large-scale industrial use of an immobilized enzyme
(Vieille and Zeikus 2001).
The activity of both free and immobilized enzyme
was measured using spectrophotometer at 410nm.
For technological and economical reasons, industrial processes have to be done by using immobilized enzyme
can provide reproducible digestions in minutes through automation, optimized buffers and trypsin immobilized enzyme
Soluble and immobilized enzyme
has been evaluated for the hydrolysis of lactose from milk and whey in batch process at 50[degrees]C.
Apart from the classical electrochemical (usually amperometric) biosensors based on the immobilized enzyme
, biosensors that are based on the immunochemical principle with the piezoelectric detection and the possibility of the antigen immobilization on the surface of a piezoelectric crystal prevail.
2 ml of 3 M acetic acid when the immobilized enzyme
The specific activity of the free was compared to that of the immobilized enzyme