IPTG


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IPTG

Abbreviation for isopropylthiogalactoside.
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IPTG

abbrev. isopropyl 3-D-thiogalacto-pyranoside. A synthetic inducer of (3-galactosidase activity in various BACTERIA. IPTG is used with the chromogenic substrate ‘X-gal’ to distinguish between recombinant and non-recombinant bacterial colonies, using cloning strategies based on INSERTIONAL INACTIVATION of the lacZ gene (of the LAC OPERON ), which encodes
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
And all of them using IPTG as the inducer, also, owing to protease deficiency in BL21 (DE3) is suitable for expression of recombinant proteins (19).
At this point induction was made with inducer (IPTG or lactose) and transferred the cells at 37C for the expression of recombinant protein.
Results from SDS-PAGE analysis of expression products showed that the Pil[Q.sub.380-706] protein expressed 4 hr after induction with IPTG. The expression product of the protein was approximately 37 kDa in molecular size (Figure 2).
The recombinant pQE 501 clones were confirmed by release of the insert by double digestion with BamHI and PstI restriction enzymes and on induction with IPTG for 4 hours appearance of 18.5 kDa protein band.
The small one is present even in noninduced cells but the large one is produced after IPTG stimulation only (Figure 4).
coli BL21 after inducing expression by IPTG at 0.1 M final concentration for 3 h and 4 h.
The optimization studies demonstrated the maximal production of recombinant phytase, when the recombinant cells were induced with 1.4 mM IPTG with the post induction time of 6 hours.
Kanamycin was obtained from duchefa, Taq polymerase, 2x PCR master mixes, 1 kb DNA Ladder, Nhel and Xhol Restriction Enzymes, T4 DNA Ligase, IPTG (isopropyl-P-D thiogalactopyranoside) and Protein ladder were obtained from fermentas.
One mM IPTG was added at mid-exponential growth phase ([OD.sub.600] [approximately equal to] 0.6) and incubated at 30[degrees] C, 180 rpm for 6 hours.
Agarose, Ammonium persulphate, Acryl amide, Bovine serum albumin, Bisacryl amide, [beta]-mercapto--Life technology USA ethanol, Bromophenol blue, Calcium Chloride, Coomassie brilliant blue 250, Magnesium Chloride, Sodium Chloride, Potassium Acetate, Tween-20, Isopropyl thiogalactosepyranoside (IPTG), X-gal, lysozyme, Sodium dodecyl sulphate, Diaminobenzidine, TEMED, Glycine, Ethidium Bromide, Proteinase K, RNase, Triton X-100, PMSF, Trypan blue, Fetal calf serum, GMEM, Goat anti-rabbit IgG HRP conjugate, Rabbi t anti-goat IgG HRP conjugate, Dialysis tube and Antibiotics (ampicillin and kanamycin) were obtained from, Sigma Chemicals, USA.
Expression of His-tagged MDH (His-MDH) was induced by 1 mM IPTG, analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie blue staining.