Comparative mapping of sheep inhibin subunits alpha (INHA) and beta B(INHBB
) to chromosome 2 in goat by FISH.
Moreover, gremlin 1 (GREM1) and inhibin beta B subunit (INHBB
) that encode ligands of the TGF-[beta] superfamily were among the most highly expressed transcripts in CTC-MCC-41 cells.
Inhibin alpha (INHA) gene encodes the alpha subunit of inhibin A and inhibin B, which together with their corresponding beta units (INHBA and INHBB
), a class of dimeric glycoproteins, also belong to the TGF-[beta] superfamily(38).
Yang, "RNAi-mediated knockdown of INHBB
increases apoptosis and inhibits steroidogenesis in mouse granulosa cells," The Journal of Reproduction and Development, vol.
Transcripts that increase 1-2 hr after acute dosing of estrogenic compounds are components of the [E.sub.2] responsive "early gene cluster," which includes Fos and Inhbb (inhibin beta-B) (Hewitt et al.
At 2 hr, the [E.sub.2]-regulated genes Fos and Inhbb were significantly up-regulated in WT mice treated with either [E.sub.2] or D3 (p < 0.01 for Fos, and p < 0.05 for Inhbb) compared with vehicle (Figure 3A).
Similarly, we found that D3 stimulated the expression of early-phase genes (Fos and Inhbb) in a fashion similar to that of [E.sub.2].
, Mad2l1, and Ppp2r2c are all transcripts we have previously demonstrated to be increased in the uterus 2 hr after [E.sub.2] treatment in WT but not in KIKO or [alpha]ERKO mice (Hewitt et al.
The mRNA levels of the estrogen-inducible genes Grebl, Inhbb
, and Fos were increased after treatment with [E.sub.2] alone; however, a 30-min pretreatment with MAA reduced the [E.sub.2]-mediated stimulation of each gene (Figure 3B).