A commercial ELISA in 96-well plate was done, Rat IL4
or IL10 Platinum ELISA (eBioscience), following the manufacturer's protocol.
In response to the four proteins, IL4
, IFN[gamma], and IL10 were produced in the same way (P = 0.605, P = 0.362, and P = 0.107, for Pru p 3.01, Pru p 3.02, and Pru p 3.03, resp.) with more production of IL4
than IFN[gamma] (Figure 5(a)).
In AVH-E patients IFN-[gamma], CD25, IL4
, IL6, IL17A, CD95, CTLA-4, PD1, PD1L, GITR, CD39, CD70, CD137, CD71, CD103, CCR4, CCR7, IL10, and TGF-[[beta].sub.1] genes were upregulated.
The Array Kits are designed for the simultaneous detection of up to 12 pro- and anti-inflammatory cytokines and chemokines (IL1[alpha], IL1[beta], IL2, IL4
, IL6, IL8, IL10, IL12, IL17A, IFN[gamma], TNF[alpha], and GM-CSF).
Caption: Figure 3: Cytokines involved in various T-cell responses, IL18 (a), IL10 (b), and IL4
(c), with similar induction in SLE patients and in HCs upon EBNA1 stimulation.
Tyutina, "Immunological parameters and gene polymorphisms (C-590T IL4
, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia," International Journal of Circumpolar Health, vol.
(a-c) The mRNA expression of cytokines and chemokines including IL1[alpha], IL1[beta], IL4
, IL6, IL8, IL10, IL12A, IL12B, IL13, IL17A, IL17B, IL18, IL23A, IL33, TNF[alpha], IFN[beta], and TGF[beta] were determined by qRT-PCR assays.
The full factorial ANOVA with all three polymorphisms, SOB, and their interactions as between-subjects factors yielded a significant effect of the IL4
X SOB interaction on the SPQ total score, F(1,262) = 7.45, p = 0.007,and [[eta].sup.2] = 0.028.
Studies on cell cultures from asthmatic patients have shown that increased Th2 cell numbers and higher production of IL4
and IL14 cytokines result in decreased barrier integrity [7, 8].
As shown in Figure 4, the expression levels of IL1, IL8, and TNF-[alpha] were significantly upregulated, whilst IL4
, IL6, IL10, IL12, and IFN-[gamma] were significantly downregulated, thus providing further indication that infection with E.
Selected cytokines related to acute and chronic inflammation, IL6, IL12, IFN-[gamma], TNF-[alpha], IL2, IL4
, IL5, IL13, IL10, TGF-[beta]11, IL17A, and G-CSF, were investigated by RT-PCR (as above reported) and by ELISA (Multi-Analyte ELISArray kit, Qiagen, Milan, Italy) as previously described .
concentrations correlated inversely with the lung function parameters [FEV.sub.1]/FVC (p=0.043; r=-0.287; Figure 3(c)) and [FEV.sub.1-post] (p=0.030; r=-0.307; Figure 3(d)).