NTS is delighted to announce their upcoming IL3
breakfast briefing event, sponsored by Egress Software Technologies, taking place at the NTS offices in Shipley, Yorkshire, on 2nd April.
In contrast, iL3s are nonfeeding (the mouth is completely sealed), migrate away from faeces, are environmentally resiliant and can survive for months in this developmentally arrested state until contact with a suitable host.
Both iL3s and parasitic adults elicit immune responses that protect against subsequent infection, suppressing larval establishment as well as parasite survival and fecundity (reviewed by Dawkins 1989; Nawa et al.
These reduce the size and duration of subsequent challenge infections, and the extent of this protection is dependent on the number of iL3s in immunizing inocula (e.
To determine whether developing larvae were responding to effects of immune status per se, we varied the number of iL3s in inocula to generate variation in larval density in faecal cultures while simultaneously varying host immune status.
In percutaneous infections, rats were anaesthetized by intraperitoneal injection with 37 to 50 mg/kg body weight of pentabarbitone (Sagatal, Rhone Merieux), a patch of fur clipped from the flank and iL3s placed directly on dampened skin.
After three days at 19 [degrees] C, worms in a culture of infected rat faeces have matured into iL3s or free-living adults.
Six rats were infected by subcutaneous injection of 500 iL3s and infections monitored from day 7 postinfection (PI) onwards.