Natural Tregs have been observed to predominantly infiltrate tumour masses especially in the early phase of tumour progression (48) both in animal models and various human malignancies viz., lung, breast, ovary, lymph nodes of human metastatic melanoma (49-54) and in cervical cancer (35,40,45,47) and play a major role in tumour immune evasion by strongly suppressing IL2
production and proliferation of antigen specific T cells (49).
Oh, "Down-regulation of Tet2 prevents TSDR demethylation in IL2
deficient regulatory T cells," Biochemical and Biophysical Research Communications, vol.
Further we stimulated our cells with IL2
, a cytokine known to be important for Treg survival while Costantini and colleagues only used CD3/CD28 stimulation.
 Human genes: IL6, interleukin 6 (interferon, beta 2); TNF, tumor necrosis factor (TNF superfamily, member 2); IL2
, interleukin 2; IL3, interleukin 3 (colony-stimulating factor, multiple); IL5, interleukin 5 (colony-stimulating factor, eosinophil); CSF2, colony stimulating factor 2 (granulocyte-macrophage); IL8, interleukin 8.
In combination with IL2
and IL10, pansorbin provoked a strong boost in B cell proliferation and in Ig and cytokine production.
We obtained a similar result by adding transcripts of interleukin-2 (IL2
) that were not detectable in unstimulated whole blood (data not shown).
 revealed that fewer EBV-specific cytotoxic T lymphocytes (CTLs) are able to secrete IFN[gamma], TNF[alpha], and IL2
Nagarkatti, "The role of IL2
in the activation and expansion of regulatory T-cells and the development of experimental autoimmune encephalomyelitis," Immunobiology, vol.
The Array Kits are designed for the simultaneous detection of up to 12 pro- and anti-inflammatory cytokines and chemokines (IL1[alpha], IL1[beta], IL2
, IL4, IL6, IL8, IL10, IL12, IL17A, IFN[gamma], TNF[alpha], and GM-CSF).
The specific cytokine groups whose differential expression were most closely associated with severe sepsis included the T-cell homeostatic cytokines IL2
and IL7 ; cytokines modulating the interaction between innate and adaptive immunity, namely, IL23 and IL27 ; and cytokines enhancing bactericidal activity, namely, TNF[alpha] and IFN[gamma] .
In AVH-E patients IL2
, CD25, IL4, PD1, CD103, CCR4, IL10, and TGF-[[beta].sub.1] genes were upregulated (Figure 4(b)), while IFN-[gamma], TNF[alpha], IL17A, CD95, PD1L, CD70, and CD69 genes were downregulated (Figure 4(c)).