, IL13, IL16 genes play an important role on the survival of NSCLC patients (30, 31).
As CD80 and CD86 are expressed by activated macrophages, we used qRT-PCR to check the expression of IL12A
and IL12B genes encoding for the M1 cytokine IL12.
Meanwhile, we found that coculture upregulated the mRNA levels of IL12A
and IL17A, markers for activated APCs and Th17, respectively, in PBMCs (Figure 3(a)) and mRNA levels of CD40, CD80, CD86, ICOSLG, and IFNy in SGECs (Figure 3(b)).
Association of IL12A
Expression Quantitative Trait Loci (eQTL) With primary biliary cirrhosis in a Chinese Han population.
Current N[O.sub.2] and [PM.sub.10] exposures at 16 y of age were linked to differential expression of IL1 b, IL12A
, as well as IL13.
Using the data from macrophages and fibroblasts, PathGen linked IL-27 to regulation of IL1[beta], TNF, and IL-6 through the IL12a
and NF-[kappa]B/Rel pathways and to TRAF2 potentially through MDFI.
Van Hagen et al., "Genome-wide association study in an admixed case series reveals IL12A
as a new candidate in Behcet Disease," PLoS ONE, vol.
Among these, IL12A
, IL12RB2, IRF5, TNPO3, DENND1B, SPIB, TNFAIP2, CXCR5, CLEC16A, RAD51L1, IRF8, RPS6KA4, MAP3K7IP1, TNFRSF1A, PLCL2, ELMO1, and ARF7 played an important role in the morbidity of PBC in European ancestries, while TNFSF15 and POU2AF1 were unique in Japanese ancestries, and CD80, IKZK3, Il7R, NFKB1, and STAT4 were proved to be associated with PBC in both races.
Changes in IL12A
methylation pattern in livers from mice fed DDC.
A third SNP (rs17810546) located in the interleukin 12A gene (IL12A
) was significantly associated with enthesitis-related arthritis.
IL-12 is a heterodimer comprised of two subunits: a 35kDa (p35) light chain encoded by IL12A
gene and a 40kDa heavy chain (p40), encoded by IL12B gene.
However, these studies often uncovered different loci in the same signaling pathways across ethnicities; [8-12] genetic variants of the IL12 [alpha]-chain (IL12A
) and IL12RB2 genes were associated with disease susceptibility in Caucasian studies, [8-11] but such associations have not been confirmed in the Japanese .