The percentage was calculated as follows: (number of Iba1
- -positive microglia/total number of DAPI) x 100% (Figure 4).
Kita et al., "Bone marrow-derived cells expressing Iba1
are constitutively present as resident tissue macrophages in the mouse cochlea," Journal of Neuroscience Research, vol.
Immunochemistry was used to determine the GFAP (a) and IBA1
(b) expression levels in the NS- or A[beta]- treated hippocampus of rats after inhaling 30% [O.sub.2] and 2.5% sevoflurane at the indicated times.
(a) Double immunofluorescence analysis revealed the colocalization of TLR2-immunoreactive cells (green) with the microglia marker Iba1
(red, colocalization marked with white arrows); (b) no colocalization of TLR4 (green) with Iba1
(red) was observed (n = 2).
antibody is a specific microglial marker and is up-regulated during the activation of microglia.
Real-time RT-PCR analysis of two genes recognized as glial cell responders following TBI showed that expression of IBA1
did not change (Figure 3).
It was colocalized with the microglia marker Iba1
in the cortex.
We found a higher number of GFAP (a and b) and Iba1
(c and d) reactive cells in hippocampi of grafted animals in comparison to sham rats (e).
Kohsaka, "Microglia-specific localisation of a novel calcium binding protein, Iba1
," Molecular Brain Research, vol.
Sections were then blocked in 2% (v/v) normal donkey serum and incubated in primary antibodies including rabbit antibody against rabbit polyclonal TLR2, mouse monoclonal NeuN (1:400, Millipore, Billerica, MA, USA), and mouse monoclonal GFAP and goat polyclonal Iba1
(1:200, Abcam, Cambridge, UK).
(2015) observed marked induction of microglia activation markers (OX-42, Iba1
, and P-p38), proinflammatory cytokine IL-6, NMDA receptor subunit NR2B and BDNF in spinal cord, and dorsal root ganglia caused by chronic constriction injury to the sciatic nerve.
G-Rg1 could decrease the level of IBA-1, GFAP, EhpA, and EhpB protein expression, IBA1
, GFAP, EhpA, and EhpB positive cells, phosphorylated p38, COX-2, and PGE2 proteins, TNF-[alpha], IL-1[beta], and the oligomerica-synuclein expression in the SNpc [16-18, 21, 22].