Influence of DNA extraction method, 16S rRNA targeted hypervariable regions
, and sample origin on microbial diversity detected by 454 pyrosequencing in marine chemosynthetic ecosystems.
As part of an ongoing stock enhancement program for red snapper, Lutjanus campechanus, in the northern Gulf of Mexico, we assessed the potential for using a hypervariable region
sequence of the mitochondrial DNA control region (mtCR) as a genetic tag.
The near-infinite range of antigen specificity is determined by changes in short, looping sequences called hypervariable regions
, so researchers can concentrate on these smaller bits of the amino acid sequence.
PCR was used to amplify a 591 bp fragment in the D-loop hypervariable region
in mtDNA by the following primer pair (Forward: 5'-AGGACTACGGCTTGAAAAGC -3' and Reverse: 5'-ATGTGCCTGACCGAGGAACCAG-3').
315], 7CT5C) in hypervariable region
(HV); polyC 16 184, nt 16184-16193 ([sup.
The primers forward: 5'-AGGACTACGGCTTGAAAAGC-3' and reverse: 5'-ATGTGCCTGACCGAGGAACCAG-3') were used to PCR amplify a 591 bp fragment of the D-loop hypervariable region
A sensitive denaturing gradient-gel electrophoresis assay reveals a high frequency of heteroplasmy in hypervariable region
1 of the human mtDNA control region.
The forward (L15952: 5'-CCCAGGCAAGAGGTAATGTA-3') and reverse primers (H198: 5'-TGTCCTGTGACCATTGACTG-3') were designed to amplify 588 bp from the hypervariable region
Therefore, we sequenced PCR-amplified fragments of the RNA polymerase b-subunit gene (rpoB), citrate synthase gene (gltA), filamenting temperature-sensitive mutant Z gene (ftsZ), VirB type IV secretion system VirB4 component gene (virB4), hypervariable region
2 of the 16S23S rRNA intergenic spacer region (ISR), and ssrA (online Technical Appendix Table 2).
Primer sites for group-specific amplification have traditionally involved the hypervariable region
at the 5' end of exon 2.
SuperAntibody properties can be engineered into any antibody through sites within the framework of the hypervariable region
ORF1 polyprotein contained motifs consistent with a methyltransferase, a peptide containing a Y domain, a papain-like cysteine protease, a peptide with a hypervariable region
(HVR), a helicase, and an RNA-dependent RNA polymerase.