Whereas, complex 3 displays slight hyperchromic effect
and hypsochromic shift.
Otherwise, a hyperchromic effect
is a sign of a change in the tertiary structure of the biopolymer promoted by the compound and could be evidence of DNA scission; this effect is caused by the major exposure of the nitrogen base pairs when unrolling or splitting [28-30].
The major peak of Mn-porphyrin is located at 477 nm, the highest intensity of the gold plasmon is positioned at 525 nm, and the plasmonic band of the MnTTPCl/n-Au hybrid is strongly bathochromically shifted to 590 nm, widening in the wavelength range of 480-750 nm and manifesting a hyperchromic effect
. These three features of the hybrid nanomaterial recommend it for optoelectronic applications .
The peak associated with [pi] [right arrow] [[pi].sup.*] transition centered at 365.97 nm also showed a red shift with a hyperchromic effect
. Formation of isosbestic points at 280, 300, 370, and 440 nm due to shifting of the peaks indicates the involvement of hydroxyl group of naphthyl ring and the imine nitrogen in complexation.
The observed hyperchromic effect
in the absorption spectra beyond 600 nm of design (1) samples can be attributed to the decrease of the sample transmission by increasing nanosilica concentration to about 79% of that of pure PMMA as clarified by the inset in Figure 4.
Spectral subtleties such as hyperchromic effect, hypsochromic red shift and blue shift may predict the behavior of the drug towards ds.DNA [18, 19, 21].
The observed hypsochromic and hyperchromic effects in the absorbance pointed to strong interactions between the host (ds.DNA) and the guest, FC-2.
The intercalative binding of drug with DNA is associated with hypochromic effect and a large red shift while the groove binding and electrostatic interaction may be associated with hypochromic and/or hyperchromic effect. Hypochromic and hyperchromic shifts are the spectral features for the double helical structure of DNA.
Both the hypochromic and hyperchromic effects were observed along the series.
was observed along the series except for the compound 4, which could not show remarkable DNA binding activity.
On addition of increasing concentration of DNA hyperchromic effect
was observed but at the same time it could not be conclusively decided that the change in absorption in a particular region was due to addition of DNA or due the interaction of DNA with compounds 1-3.
While in the presence of increasing concentration of CTAB in a fixed concentration of 5-FU, the signal at 206 nm showed increase in absorbance (hyperchromic effect
) accompanied with red shift (bathochromic effect), generation of another signal at 296 nm and an isosbestic point.