Mason Sones, Jr., a pediatric cardiologist at the Cleveland Clinic, inadvertently power injected 20 mL of hypaque
via an NIH catheter into the right coronary artery, as a result of catheter whiplash into the ostium of the vessel during root aortography.
Andersen, "Single step separation of red blood cells, granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll Hypaque
," Journal of Immunological Methods, vol.
Modification of neurotoxicity of methylglucamine diatrizoate (hypaque
75 per cent) by intra-arterial instillation of vasoactive drugs.
Posteriormente, se centrifugo la muestra para obtener el plasma y la fraccion celular, a partir de la cual se separaron celulas mononucleares de sangre periferica, utilizando el gradiente de densidad de Ficoll Hypaque
(GE Healthcare Bio-Sciences Corp., Piscataway, NJ, Estados Unidos).
Neutrophils were isolated using a standard method by dextran sedimentation and centrifugation in a Ficoll Hypaque
gradient (Boyum 1968).
En un tubo falcon se adicionaron 3 ml de Ficoll-Hypaque (Ficoll Hypaque
Separation of MNCs: Mononuclear cells (MNCs) were isolated from adult human peripheral blood by gradient centrifugation at 2500 rpm for 30 minutes on Ficoll Hypaque
Stromal Vascular Fraction (SVF) was isolated and cultured in DMEM culture medium (GIBCO) containing 10% fetal bovine serum (GIBCO) using Ficoll hypaque
(Biosera) gradiant centrifugation.
peripheral blood mononuclear cells (PBMC) Heparinized venous blood samples were obtained from patients and controls, and PBMC were isolated using the Ficoll Hypaque
density-gradient centrifugation technique.
Density gradient selection using Ficoll Hypaque
(FH)-based gradients remains the most common approach.