Hypaque

Hypaque®

Diatrizoate, see there.
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Fresh peripheral blood mononuclear cells were prepared from heparinised blood using standard Ficoll Hypaque separation and cultured for 24hrs at 37C for interferon gamma analysis using ELISPOT assays (BD Pharmingen) according to the manufacturer's instructions.
Density gradient selection using Ficoll Hypaque (FH)-based gradients remains the most common approach.
Neutrophils were isolated with a standard method of dextran sedimentation prior to hypotonic lysis of erythrocytes and to centrifugation in a Ficoll Hypaque gradient The purity of the neutrophils preparation was >97% and viability measured by tryptan blue exclusion was >98%.
From the l0 ml samples the plasma and peripheral blood mononuclear cells (PBMC) were separated by the density gradient technique using Ficoll hypaque and stored in multiple aliquots at -60[degrees]C until tested.
The spores were purified by centrifugation through 58% Hypaque 76 (NYCOMED, Inc.
This is done with density centrifugation over Ficoll Hypaque.
Peripheral venous blood from the patients with AMI (20 mL) or with stable CHD (5 mL) was layered on an equal volume of Ficoll Hypaque (Shanghai Biotech) and centrifuged at 1500g for 20 min at 25 [degrees]C.