hybridoma

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hybridoma

 [hi″brĭ-do´mah]
a somatic cell hybrid formed by fusion of normal lymphocytes and tumor (lymphoma) cells. B cell hybridomas are the most useful source of monoclonal antibodies; the hybridoma cells are able to produce antibody like the normal parent and to proliferate indefinitely in culture like the parent tumor cells.

hy·brid·o·ma

(hī'brid-ō'mă),
A tumor of hybrid cells used in the in vitro production of specific monoclonal antibodies; produced by fusion of an established tissue culture line of lymphocyte tumor cells (for example, mouse plasmacytoma cells) and specific antibody-producing cells (for example, splenocytes from specifically immunized mice). Fusions are accomplished by use of polyethylene glycol or other methods.
[G. hybris, violation, wantonness, + -ōma, tumor]

hybridoma

(hī′brĭ-dō′mə)
n.
A cell that is produced in the laboratory by fusing an antibody-producing lymphocyte (which does not readily divide) with a nonantibody-producing cancer cell (which divides rapidly). The hybridoma proliferates and produces a continuous supply of a specific monoclonal antibody.

hy·brid·o·ma

(hī'brid-ō'mă)
A tumor of hybrid cells used in the production in vitro of specific monoclonal antibodies; produced by fusion of an established tissue culture line of lymphocyte tumor cells and specific antibody-producing cells.
[G. hybris, violation, wantonness, + -ōma, tumor]

hybridoma

A cell line (CLONE) formed by the fusion of two or more different types of cells, such as a B LYMPHOCYTE and a lymphoid tumour cell (myeloma). Malignant tumours can be immortal so a hybridoma, suitably nourished, can continue permanently exercising the function of the B lymphocyte to produce specific antibodies (MONOCLONAL ANTIBODIES).

hybridoma

a cell made by fusing an antibody-producing B-CELL with a CANCER cell. The cancer cell contributes the ability to divide indefinitely, while the antibody cell contributes the ability to synthesize large amounts of a single antibody A hybridoma CLONE forms as a result of division of this hybrid, and can be maintained indefinitely in culture, producing large amounts of pure antibody The antibodies produced by a specific hybridoma clone are identical, they are MONOCLONAL ANTIBODIES.
References in periodicals archive ?
Hybridoma cell line production of monoclonal antibodies is static; so once a desirable cell line is identified, the immunoreagent is of constant and known affinity (Goding, 1986).
americana allergen, secreted from the hybridoma clone 38G6 [protein purified from American CR extract that bound to this MAb was shown to be an allergen (Sookrung et al.
Membranes were incubated for 30 min at room temperature in blocking buffer [5% nonfat dry milk in Tris-buffered saline (TBS) containing 20 mM Tris-HC1, 500 mM NaC1, pH 7.5], followed by a 2-h incubation in hybridoma supernatant diluted 1:4 in blocking buffer containing 0.05% Tween 20.
These antibodies were obtained from a culture supernatant solution of hybridoma cells that was produced by a cell fusion procedure between myeloma cells and antibody-producing spleen cells.
ImmunoPrecise is currently in negotiations with several top 10 global pharmaceutical companies investigating the use of ImmunoPrecises three full-service platforms, its advanced hybridoma platform, its proprietary and high demand B-cell selectplatform, and its phage display library platform.
Anti-Plexin C1 monoclonal antibodies were purified from hybridoma supernatants on AKTA-Purifier Chromatography (GE, Massachusetts, USA) using protein G affinity column (HiTrap protein G, GE).
Three hybridomas (6D-1G, 6D-4E, and 6D-7C) were maintained in culture and the antibodies produced were able to recognize cultured adenovirus (prototypes) 2, 3, 5, and 41 (Fig.
Culture supernatants from clonal hybridoma lines of interest, diluted mouse anti-ferret Ig (M[alpha]F-Ig) mAb or mouse IgG1,[kappa] (Biolegend, Cat #401402), were serially diluted in ELISA blocking buffer and plates incubated for 90 min at 37[degrees]C.
To exclude the possibility that our surprising results were due to an unknown bias in the hybridoma cloning, we carried out RNase protection assays in which C57BL/6 (B6) type [sup.32]P-labelled antisense V[alpha]14J[alpha]18 mRNA derived from one of the hybridomas was hybridized with total mRNAs (sense) from normal B6 thymocytes.
(11.) Goding JW: Antibody production by Hybridomas. J Immunol Meth, 39: 285-294 1980.