housekeeping gene


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housekeeping gene

n.
Any of a group of genes that are expressed in all cells and code for proteins that are essential for basic cell growth and metabolism.

housekeeping gene

A gene expressed in nearly every cell and every tissue of an organism, i.e., one that encodes a protein fundamental to cellular activity throughout the organism.
See also: gene
References in periodicals archive ?
Because these samples had one target gene CT value >30 and a CT value for the housekeeping gene <30, [DELTA]CT values were positive and were located under these cutoffs in the samples with failed expression.
We report here an RT-PCR-based assay combining the determination of expression of both human IMPDH isoenzymes based on normalization to a housekeeping gene index.
Usually, the well-known housekeeping genes are chosen such as the glyceraldehydes-3-phosphate dehydrogenase (GAPDH), [alpha]-actins (ACTB), hypoxantine phosphoribosyltransferase (HRPT), [beta]-tubulin (TUB), elongation factor 1 alpha (EF1A) and 18S, 28S rRNA (Shu et al.
The relative p53-like gene expression was evaluated using 18S rRNA as a housekeeping gene.
Many housekeeping genes do show different expression patterns under different experimental conditions and the influence of physical exercise on housekeeping gene expression is not well studied (Jemiolo and Trappe, 2004; Mahoney et al.
The standard curves were prepared for both tested and housekeeping gene, GAPDH (GenBank: U48832).
For each sample, the quality of DNA extraction was verified by real-time PCR for a housekeeping gene encoding p-actin (7).
We also quantified the transcripts of the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous control to normalize each sample using sense and antisense primers, 5' CCA TCA CCA TCT TCC AGG AGC 3' (positions 278-298) and 5' GGA TGA TGT TCT GGA GAG CC 3' (positions 663-682), respectively (Tokunaga et al.
ACTIN, a housekeeping gene with expression unchanged during THH treatment, was chosen to serve as a control.
mRNA from both the gene of interest and a housekeeping gene permits the