housekeeping gene


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housekeeping gene

n.
Any of a group of genes that are expressed in all cells and code for proteins that are essential for basic cell growth and metabolism.

housekeeping gene

A gene expressed in nearly every cell and every tissue of an organism, i.e., one that encodes a protein fundamental to cellular activity throughout the organism.
See also: gene
References in periodicals archive ?
To show the variation of each housekeeping gene under the toxicity stress in whole bodies or midguts, the most stable gene, EF1[alpha], was used as the reference to normalize the expressions and to calculate the fold of change of expression of each gene.
A set of 8 different RNA tumor markers (Table 3) containing putative as well as established bladder cancer markers and the housekeeping gene GAPDH were quantified as specified in Fig.
Relative expression results may be influenced by interindividual differences in housekeeping gene expression.
In the UROtsa cell line, hsp 27 mRNA was highly expressed, with mRNA being detected 10-15 cycles earlier in the PCR than were the g3pdh housekeeping gene (Rossi et al.
Hence three different software tools were used to validate the stability of the selected housekeeping genes in different developmental stages (1 d, 2, 4, 6 and 8 months old) of Zi geese tissues (heart, liver, kidney, muscle, ovary) using real-time qRT-PCR.
Total RNA was isolated from confluent UROtsa cells grown on both serum-containing and serum-free growth medium and used to determine the basal expression of the respective heat shock protein genes relative to the glyceraldehyde 3-phosphate dehydrogenase (g3pdh) housekeeping gene using RTPCR technology as described previously (23-25).
The sequence and amplicon sizes of the primers are shown in Table 1 with 18S rRNA as the housekeeping gene.
Pre designed primers and probes for c-Myc gene were selected and purchased from Applied Bio System and for data normalization, GAPDH housekeeping gene primer was custom designed using primer express software (ABI) (Table II).
Apoptotic effects by different glucose level were determined by real-time, reverse transcription-PCR and GAPDH was use as the endogenous housekeeping gene (D, E).
Hypoxanthine guanine phosphoribosyltransferase (HPRT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as housekeeping gene and data were normalized relative to the expression level of the HPRT gene.
The disease occurs when a faulty housekeeping gene fails to remove cell waste, like lipids and cholesterol.