DNA sequencing

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DNA sequencing

n.
The determination of the sequence of nucleotides in a sample of DNA.

base-sequence analysis

A generally automated method for determining the order of nucleotide bases in a nucleic acid (DNA or RNA).

DNA sequencing

The determination of the sequence of base pairs in a length of DNA.

DNA sequencing

determination of the order of NUCLEOTIDES in a DNA molecule or part thereof. A number of techniques is available to determine a DNA sequence, for example the chain-terminator DNA sequencing procedure (see DIDEOXYRIBONUCLEOTIDE). Generally such techniques rely on the power of polyacrylamide gels to resolve NUCLEIC ACID molecules that differ in length by only a single nucleotide. Ladders of DNA fragments are formed on the gels following ELECTROPHORESIS. Both manual and automated methods of sequencing are available.
References in periodicals archive ?
There are many alignment tools designed specifically to handle high-throughput sequencing reads (see Table 1).
Stevens, "Antarctic eukaryotic soil diversity of the Prince Charles Mountains revealed by high-throughput sequencing," Soil Biology and Biochemistry, vol.
High-throughput sequencing was conducted using the platform of Shanghai Biotechnology and the HiSeq 2500 sequencer (Illumina).
Then, high-throughput sequencing was used to assess the taxonomic composition of the gut microbiota in CHD patients.
To process full-measure high-throughput sequencing information sets proficiently, a few calculations taking into account word tallying are proposed.
The web page provides scientists with optimized ChIP methodologies for qPCR, high-throughput qPCR, sequencing, high-throughput sequencing and enzymatic chromatin shearing using Chromatrap kits.
IntegraGen will be responsible for the technical and operational implementation of high-throughput sequencing activities for use by the microbiology experts at the NRC at the Institut Pasteur.
High-throughput sequencing (HTS) is also at the forefront of screening samples for presence of rare, invasive, and threatened or endangered species (e.g., Mahon eta/., 2014.
The biggest expense in sequencing a human genome now is the cost of storing the information, says Scott Kahn, vice president of commercial enterprise informatics at Illumina, a San Diego biotech company specializing in high-throughput sequencing. Someday soon, he says, it may be cheaper to resequence a person's genome each time the data are needed than to store the information as is and 0s.
Performance comparison of benchtop high-throughput sequencing platforms.
In this study, using high-throughput sequencing techniques, the team led by researchers from Queen Mary, University of London, sought to identify new variants, including rare and potentially high risk ones, in 25 previously identified risk genes in a sample of nearly 42,000 individuals (24,892 with autoimmune disease and 17,019 controls).
It will also boast state of the art laboratory facilities, including high-throughput sequencing equipment.