19) A new method, combining seminested polymerase chain reaction with heteroduplex analysis
, was used by Oehadian et al (20) to detect FL cells in peripheral blood.
Identification and characterization of new BoLA DRB3 alleles by heteroduplex analysis
and direct sequencing.
Alternate methods can been used to increase the detection sensitivity of melting assays, such as decreasing the PCR product length (10), calibration with an internal oligonucleotide duplex (13), quantitative heteroduplex analysis
after mixing with a known genotype (25), or the use of unlabeled probes (14, 15) or snapback primers (26, 27).
Applications of heteroduplex analysis
for mutation detection in disease genes.
The DNA Toolbox has been used previously to assess the SNP scanning sensitivity of heteroduplex analysis
(7) and SSCP analysis (17).
Optimized PCR fragments for heteroduplex analysis
of the whole human mitochondrial genome with denaturing HPLC.
In this case, a 2-stage mixing strategy allows heteroduplex analysis
to be used instead of melting temperature analysis for the detection of homozygous changes.
Polymerase chain reaction products can be detected by agarose and polyacrylamide gel electrophoresis, single-strand conformational polymorphism, heteroduplex analysis
, denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), and capillary gel electrophoresis.
of T-Cell Receptor [Gamma] Gene Rearrangement
In cases in which homozygotes are difficult to distinguish, amplicon-melting analysis can still provide complete genotyping information via their mixing with a known homozygote followed by quantitative heteroduplex analysis
The PCR products were subjected to mutation screening by single-strand conformation polymorphism and heteroduplex analysis
Capillary and microchip electrophoresis for rapid detection of known mutations by combining allele-specific DNA amplification with heteroduplex analysis