heteroduplex

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het·er·o·du·plex

(het'ĕr-ō-dū'pleks),
1. A DNA molecule, the two constitutive strands of which derive from distinct sources and hence are likely to be somewhat mismatched.
2. A DNA-RNA hybrid.
[hetero- + L. duplex, two-fold]
Farlex Partner Medical Dictionary © Farlex 2012

het·er·o·du·plex

(het'ĕr-ō-dū'pleks)
1. A DNA molecule, the two constitutive strands of which derive from distinct sources and hence are likely to be somewhat mismatched.
2. A DNA-RNA hybrid.
[hetero- + L. duplex, two-fold]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

heteroduplex

a DUPLEX DNA in which the two strands do not show COMPLEMENTARY BASE PAIRING throughout, but instead contain one or more MISMATCHES OF BASES. A heteroduplex may be formed during RECOMBINATION, for example. Compare HOMODUPLEX.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
The indirect diagnosis, the heteroduplex analysis and sequenciacion of the FIX gene were utilized for detecting these mutations.
Applications of heteroduplex analysis for mutation detection in disease genes.
Chhanabhai et al,[20] using a single set of primers to amplify TCR[Gamma] and heteroduplex analysis, reported 89% positivity in T-cell lymphoid neoplasms.
The PCR products were subjected to mutation screening by single-strand conformation polymorphism and heteroduplex analysis (7).
This form of heteroduplex analysis has focused on detection, and although it may involve a quantitative threshold, the result is essentially binary.
Earlier studies on heteroduplex analysis suggested that the kinetics of heteroduplex formation would fit a quadratic model (26, 27).
Capillary and microchip electrophoresis for rapid detection of known mutations by combining allele-specific DNA amplification with heteroduplex analysis. Clin Chem 2001;47:173-85.
In these cases, there are 2 options to retain the simplicity of closed-tube genotyping with a dye: (a) wild-type DNA can be added to all samples and quantitative heteroduplex analysis performed (20); or (b) an unlabeled probe can be included in the PCR to differentiate the homozygotes (28).
Several approaches have been developed for the analysis of cancer susceptibility genes by microchip CE, including single-strand conformation polymorphism analysis (33) and a combination of allele-specific DNA amplification with heteroduplex analysis (34-37).
These methods include single-strand conformation polymorphism analysis, denaturing gradient gel electrophoresis, temperature gradient gel electrophoresis, heteroduplex analysis, denaturing HPLC, temperature gradient capillary electrophoresis, and chemical or enzymatic mismatch detection methods (25).