illness cells/L 1 ([dagger]) GVHD, renal 110 failure 2 ([dagger]) None 540 3 GVHD 1,470 4 None 1,440 5 Renal failure 0 6 ([dagger]) CMV, GVHD 154 7 Lung metastasis, 366 thoracotomy, restrictive syndrome 8 None 1,145 * BMI, body mass index; HCT, hemopoietic cell
transplant; AML, acute myeloblastic leukemia; allo, allogenic; MMF, mycophenolate mofetil; GVHD, graft-versus-host disease; NA, not applicable; CML, chronic myeloblastic leukemia; TBI, total body irradiation; ALL, acute lymphoblastic leukemia; CSA, cyclosporine; CMV, cytomegalovirus; ATG, antithymocyte globuline.
Further immunological characterization of pathological cells in the clinical laboratory should be performed whenever a specific feature of these cells has been shown to be of clinical importance for the diagnosis (immunological features that are specific of a certain cell lineage and maturation stage), prognosis (subclassification of leukemias affecting the same hemopoietic cell
lineage according to differentiation features or phenotypic characteristics associated with certain genetic abnormalities), or monitoring of the disease (investigation of phenotypic aberrations and abnormal differentiation pathways).
Those assays quantify hemopoietic cell
proliferation (Raftos and Cooper, 1991; Raftos et al., 1991), phagocytosis (Beck et al., 1993; Kelly et al., 1993), and cellular cytotoxicity (Parrinello et al., 1993; Peddie and Smith, 1993, 1994).
In the present study, we aimed to determine the in vivo effect of injecting arabinogalactan, not only on natural killer (NK) cells, i.e., cells representing the first line of defense in vivo against virus infections and developing neoplasms, but also on a wide range of other immune and hemopoietic cell
populations in the bone marrow and spleen of mice.
Hairy cell leukemia-associated antigen (HC2) is an activation antigen of several hemopoietic cell
lineages, inducible on monocytes by IFN-gamma.
These findings indicate that the hemopoietic cell
cycle is still functional in p53-KO mice during benzene exposure, whereas in WT mice it is arrested because of alterations in the expression of cell-cycle checkpoint genes, particularly the p53 gene (Yoon et al.
We selected these inflammatory cytokines for the following features: (i) IL-6 is a pleiotropic proinflammatory cytokine that acts on many cell types including hemopoietic cells
. It has been implicated as a critical activator of myelopoiesis in response to pathogen infection and chronic inflammation ; (ii) IL-1[beta] is a potent inflammatory cytokine that mediates leukocytosis and thrombocytosis under inflammatory conditions by inducing various cytokines (i.e., granulocyte colony-stimulating factor and IL-6) .
Miyake et al., "Analysis of tolerance induction using triple chimeric mice: major histocompatibility complex-disparate thymus, hemopoietic cells
, and microenvironment," Transplantation, vol.
MUC13, a novel human cell surface mucin expressed by epithelial and hemopoietic cells
. J BiolChem, 276(21):18327-18336.
CD34 is expressed most strongly on primitive hemopoietic cells
, and is progressively lost as cells differentiate (Strauss et al., 1986).
Studies have shown that this age related loss of marrow hemopoietic cells
and decline in thymic function is reversed by growth hormone.
Aplastic anemia which may be congenital or acquired, is the result of failure of normal hemopoietic cells
of the bone marrow which results in decreased production of all types of blood cells (RBCs,WBCs platelets).