Like cellulase and hemicellulase
, multiple enzymes work together for chitin digestion.
Breaks down hemicellulose, a structural (and indigestible) component of plant cell walls.
The conserved non-catalytic 40-residue sequence in cellulases and hemicellulases
from anaerobic fungi functions as a protein docking domain.
Figure 1 Enzyme Type Aids Digestion of Plant Source ECC Units Alpha- raffinose, Aspergillus GalU Galactosidase sugars niger Amylase starch, glucose Aspergillus DU (alpha-) oryzae Bromelain proteins, poly- Ananas PU peptides cosmous Cellulase cellulose Trichoderma CU (fibers) longbrachiatum Glucoamylase starch, glucose Aspergillus AGU niger Hemicellulase
hemicellulose Aspergillus HCU fiber niger Invertase sucrose (sugar) Saccharocmyces IAU or (Sucrase) cerevisiae SU* Lactase lactose (milk Aspergillus ALU sugar) oryzae Lipase fats Aspergillus LU niger Malt Diastase starch, glucose Hordeum DP (Maltase) vulgare Papain proteins, poly- Carica papaya PU peptides Peptidase proteins, poly- Aspergillus HUT peptides oryzae Phytase phytic acid Aspergillus FTU niger Protease 3.
One unit of amylase activity was defined as the amount of enzyme required to release 1 [micro]g of maltose equivalents per minute under the assay conditions Hemicellulase
activity was determined by measuring the release of reducing sugar from the substrate (1.
Figure 1 Enzyme Type Aids Digestion Of Plant Source Alpha-Galactosidase raffinose sugars Aspergillus niger Amylase (alpha-) starch, glucose Aspergillus oryzae Bromelain proteins, polypeptides Ananas cosmous Cellulase cellulose (fibers) Trichoderma longbrachiatum Glucoamylase starch, glucose Aspergillus niger Hemicellulase
hemicellulose fiber Aspergillus niger Invertase (Sucrase) sucrose (sugar) Saccharocmyces cerevisiae Lactase lactose (milk sugar) Aspergillus oryzae Lipase fats Aspergillus niger Malt Diastase starch, glucose Hordeum vulgate (Maltase) Papain proteins, polypeptides Carica papaya Peptidase proteins, polypeptides Aspergillus oryzae Phytase phytic acid Aspergillus niger Protease 3.
Following advice from the Food Advisory Committee, the provisions permitting the use of flour treatment agents benzoyl peroxide and azodicarbonamide are being removed and hemicellulase
is being added to the list of permitted enzymes that may be used in breadmaking.
Nitrogen-free extract (NFE), hemicellulase
and cellulase was determined by calculation.
chrysosporium also produces cellulase and hemicellulase
(xylanase) enzymes (Dashtban et al.
Bacillus amyloliquefaciens was used as the inoculum, since it had been reported to produce various enzymes such as [alpha]-amylase, [alpha]-acetolactate, decarboxylase, [beta]-endoglucanase, hemicellulase
, phytase, maltogenic amylase and xylanase which possess a potential to degrade fiber (Anonymous, 2012; Diaz, 2008; Wizna et al.