As shown in Figure 3A, the percent of apoptotic
HeLa cells (quadrants 2 and 4) increased from 1% to 9.3% and 11.8% after treatment with the extract at 200 and 500 [micro]g/mL, respectively (Figure 3A; a-d).
They first established a
HeLa cell line (E6) derived from an ancestral cell line.
Besides, the proliferation, migration, and apoptosis capacity of
HeLa cells were also affected by Dkk-1 and BIO to different extents (Figure 4C to E).
Evaluation of paclitaxel effects in the pattern of expression of survival and apoptotic genes regulators in
HeLa cells. Curr Pharm Biotechnol 2016; 17(12): 1058-67.
The cell index values obtained from the real-time cell analysis system were examined following the application of 4a-d to
HeLa cells. 5, 10, 20, 40, 80, 100 and 160 [micro]M concentrations were used for all compounds.
Then immunofluorescence was used to detect the distribution of NXPH1 in DF-1 and
Hela cells. It showed that green fluorescence of NXPH1 appeared in the cytoplasm and nuclei of chicken fibroblast cells and
Hela cells, and the target protein were mainly localized in the cytoplasm (Fig.
Confocal microscopy observation of
HeLa cells transfected with SLC15A3-GFP (green) and lysosome tracker (a), ER tracker (b), endogenous Rab5 (c), endogenous Rab7 (d), endogenous Rab11 (e), and RFP-PXMP2 (peroxisome tracker) (f).
Conversely,
HeLa cells treated with piton and avocado AgNPs in concentrations up to 50 [micro]M did not show a statistically significant cytotoxic response (p > 0.05).
Henrich, "Host Cell Responses to Persistent Mycoplasmas--Different Stages in Infection of
HeLa Cells with Mycoplasma hominis," PLoS ONE, vol.
In
HeLa cells too, eugenol exhibited a concentration-dependent upregulation in the level of caspase-3 activity.
In brief,
HeLA cells obtained from American Type Culture Collection, USA, were plated in 96-well plates at a density of 1 x [10.sup.4] cells/well.
Owing to the unique properties of the N-doped CD nanoprobe with good membrane permeability and excellent biocompatibility, it was used for imaging of
HeLa cells with high discrimination.
