As shown in Figure 3A, the percent of apoptotic HeLa cells
(quadrants 2 and 4) increased from 1% to 9.3% and 11.8% after treatment with the extract at 200 and 500 [micro]g/mL, respectively (Figure 3A; a-d).
They first established a HeLa cell
line (E6) derived from an ancestral cell line.
Besides, the proliferation, migration, and apoptosis capacity of HeLa cells
were also affected by Dkk-1 and BIO to different extents (Figure 4C to E).
Evaluation of paclitaxel effects in the pattern of expression of survival and apoptotic genes regulators in HeLa cells
. Curr Pharm Biotechnol 2016; 17(12): 1058-67.
The cell index values obtained from the real-time cell analysis system were examined following the application of 4a-d to HeLa cells
. 5, 10, 20, 40, 80, 100 and 160 [micro]M concentrations were used for all compounds.
Then immunofluorescence was used to detect the distribution of NXPH1 in DF-1 and Hela cells
. It showed that green fluorescence of NXPH1 appeared in the cytoplasm and nuclei of chicken fibroblast cells and Hela cells
, and the target protein were mainly localized in the cytoplasm (Fig.
Confocal microscopy observation of HeLa cells
transfected with SLC15A3-GFP (green) and lysosome tracker (a), ER tracker (b), endogenous Rab5 (c), endogenous Rab7 (d), endogenous Rab11 (e), and RFP-PXMP2 (peroxisome tracker) (f).
Conversely, HeLa cells
treated with piton and avocado AgNPs in concentrations up to 50 [micro]M did not show a statistically significant cytotoxic response (p > 0.05).
Henrich, "Host Cell Responses to Persistent Mycoplasmas--Different Stages in Infection of HeLa Cells
with Mycoplasma hominis," PLoS ONE, vol.
In HeLa cells
too, eugenol exhibited a concentration-dependent upregulation in the level of caspase-3 activity.
In brief, HeLA cells
obtained from American Type Culture Collection, USA, were plated in 96-well plates at a density of 1 x [10.sup.4] cells/well.
Owing to the unique properties of the N-doped CD nanoprobe with good membrane permeability and excellent biocompatibility, it was used for imaging of HeLa cells
with high discrimination.