harris hematoxylin 24 l, indicated by the participants4.
Gonads were embedded in paraffin, sections cut at 5 [micro]m and stained with
Harris hematoxylin followed by eosin counterstain (Presnell & Schreibman 1997).
In contrast, the slides, used as negative control, showed only a violet color conferred by the
Harris hematoxylin (Figure 1(b)).
Some of the tissue sections from the heart, lungs, liver, and spleen were stained with alum hematoxylin (
Harris hematoxylin) and counterstained with 90% ethanolic extract of Z.
1% ACETIC ACID 10 dips
Harris Hematoxylin 10 dips [Preheated 60C] T ap water 10 dips 1% acetic acid 10 dips OG-6 10 dips 1%acetic acid 10 dips EA-50 10 dips 1%acetic acid 10 dips Methanol 10 dips Xylene 10 dips Blotting was done after each step.
In addition to examining the basic anatomy of the host-parasite relationship with
Harris hematoxylin [HH], a variety of stains were selected to test for acidic mucosubstances (alcian blue pH 1.0 [AB1], alcian blue pH 2.5 [AB2.5], toluidine blue [TB]), neutral sugars (periodic acid-Schiff [PAS]), DNA (Feulgen nuclear reaction or HCL-Schiff [FL]), proteins (ninhydrin-Schiff [NS], Ward's protein stain [WPS]), lipids (osmium tetroxide [OS]), and tyrosine/melanin (ferro-ferricyanide [FF]).
Histological sections were cut at 5 [mu]m and stained with
Harris hematoxylin followed by eosin counterstain (Presnell and Schreibman, 1997).
We used the avidin-biotin-peroxidase complex method with
Harris hematoxylin counterstain.
The sections put on glass slides, once dried, were stained with
Harris Hematoxylin and mounted in Permount.
Reaction was revealed with permanent red (DAKO[R]) and counter stained with
Harris hematoxylin (DAKO[R]).
Slides were stained with
Harris hematoxylin followed by eosin counterstain (Presnell & Schreibman 1997).
Nova Red (Vector Laboratories Canada Inc., Burlington, Ontario) was used as chromogen, and tissues were counterstained with
Harris hematoxylin (Fisher Scientific, Toronto, Ontario).
