Similarly, the expression of 3 osteoclastic gene markers for RAW 264.7 cells, osteoclast-associated receptor (OAR), tartrate-resistant acid phosphatase (TRAP), and hyaluronoglucosaminidase 1 (HYA1), was evaluated by the same method.
To explore the inhibition of osteoclastic differentiation of [Ca.sub.2]Zn[Si.sub.2][O.sub.7] ceramic nanocoating with different Zn/Ca ratios, the expression of three relative genes, OAR, TRAP, and HYA1, was assessed by RT-qPCR.
In contrast, for Zn/Ca (0.3), RAW 264.7 cells showed the lowest cell viability and inhibited the osteoclastic gene expressions of OAR, TRAP, and HYA1. In summary, the optimal ZnCa ratio of 0.3 in the [Ca.sub.2]Zn[Si.sub.2][O.sub.7] ceramic nanocoating on Ti with [Zn.sup.2+] slow release may have osteoblast-promoting and osteoclast-inhibiting effects to form a dynamic balance between osteoblasts/osteoclasts.
Osteoclastic marker genes: (a) osteoclast-associated receptor (OAR), (b) tartrate-resistant acid phosphatase (TRAP), and (c) hyaluronoglucosaminidase 1 (HYA1).