We have previously sequenced the full -length cDNA encoding the putative stress induced heat shock protein HSPA6 from Arabian camel (NCBI Gene Bank accession number HQ214118.1).
The straight-walk method was used to isolate the upstream genomic DNA sequences of the HSPA6. The isolated fragment has a length of 1417bp and after verifying the sequence, it was deposited in GenBank under accession number JX888464.
Keywords: genome walk, heat shock elements, HSPA6 gene, Camelus dromedaries, in silico.
In an attempt to start investigating components that could play a role in contributing to such characteristics, we focused on one of the cellular chaperone components; the heat shock protein A6 (HSPA6, also called HSP70B') gene from Arabian camel.
Interestingly, Wada et al (Wada et al., 2007) found a response sequence that is located upstream of the human HSPA6 gene and cloned it in tandem order in a reporter vector to use it as a sensitive tool to detect cytotoxicity.
Furthermore, the study demonstrated the occurrence of several heat shock elements (HSEs) as binding sites for the major heat shock factor including other core promoter elements like transcriptional start site for the HSPA6, canonical TATA- box, DPE, MTE, BRE, CpG island, palindromic and repeated sequences.
Only a subset of Hsp70 chaperones, namely, HSPA1A, HSPA1B, and HSPA6
, show stress-induced expression patterns, whereas the other Hsp70 family members, such as HSPA8 (often referred to as Hsc70), are expressed constitutively at baseline conditions .
Szatmary et al., "Upregulation of heat shock proteins (HSPA12A, HSP90B1, HSPA4, HSPA5 and HSPA6
) in tumour tissues is associated with poor outcomes from HBV-related early-stage hepatocellular carcinoma," International Journal of Medical Sciences, vol.