(rs14355) (2016) (26) HSPA6 (rs1042881) HSPA1L (rs2075800, rs2227956 and rs2227955) HSPA4L (rs1380154) HSPA9 (rs1042665, rs10117) Trombone et al.
The importance of HSPs in maintaining proteostasis within oocytes takes on added significance in light of evidence that oocytes obtained from aged mice exhibit a marked decrease in the gene expression of Hsp40s (Dnaja2, Dnaja4, Dnajb1, Dnajb6, Dnajb10, Dnajb11, Dnajc3, Dnajc5, Dnajc8, and Dnajc21), Hsp70s (Hspa1b, Hspa4
, Hspa8, Hspa9a, Hspa14, and Hsp70-3), Hsp90s (Hspcb), and components of the chaperonin containing TCP1 complex (Cct1-3 and Cct5) (Table 1) [3, 97].
To determine the mRNA expression of Hsp70, qRT-PCR was carried out on a Stratagene MX3005p QPCR system (Stratagene) using gene-specific primers for human HSPA4
(NM_002154, UniGene number: Hs.90093 Rn.9873, Cat number: PPH01188C) purchased from SABiosciences (SuperArray, Frederick, MD, USA).
In addition, selected miRNAs could also control DEGs involved in the innate immune response including, for example, NKTR (hsa-miR-181a-5p, -486-5p, -151-3p, and -27b-3p), KIR2DL4 (hsa-miR-146a-5p), STAT1 (hsa-miR584-5p, -27b-3p, -128-3p, and -146a-5p), STAT2 (hsa-miR143-3p), and DEGs belonging to the Toll-like receptor (TLR) pathway such as TLR2, IKBKB, JUN, REL, NAMPT, HSP90B1, HSPA4, and ARF3.
Other DEGs targeted in clusters were involved in TLR signaling including HSPA4, IKBKB, JUN, REL, S100A8, TLR2 (CL1), and ARF3 and NAMPT (CL2).
Of note, among this group of proteins, heat shock protein family A (Hsp70) member 4 (HSPA4
) and ribosomal protein S19 (RPS19) are proteins previously described as DAMPs (Supp.
Szatmary et al., "Upregulation of heat shock proteins (HSPA12A, HSP90B1, HSPA4
, HSPA5 and HSPA6) in tumour tissues is associated with poor outcomes from HBV-related early-stage hepatocellular carcinoma," International Journal of Medical Sciences, vol.