As a result, 11 of these genes had increased expression in plasma cells from patients with MM that significantly overlapped between EEF1A1, UBC, UBB, CALR, CXCR4, JUND, FOS, PIM2, JUN, GAPDH, and HSP90B1 and were previously reported as upregulated in the Munshi_multiple _myeloma data set (Figure 4).
Using in silico tools, we identified significant overlaps between EEF1A1, UBC, UBB, CALR, CXCR4, JUND, FOS, PIM2, JUN, GAPDH, and HSP90B1, which were previously reported to be upregulated in MM according to the Munishi_ MM data set (Figure 4).
As indicated in Figure 3(c), the direct or cascaded interactions of Gfra1-Ret-Kitl/Hsp90b1 (Kitl and Hsp90b1
are key genes in the PI3K-Akt pathway) were prioritized in the Gfra1-centric subnetwork.
ADAMTS9 CRIP2 ENGASE IL23A RT1-DA AHCY CTGF ERO1LB KRT20 S100A4 AMIGO3 DDIT3 ETV5 MANF SDF2L1 ANKRD37 DERL3 FGF18 MFSD2 SEC23B ATMIN DNAJB11 FOLR1 NR4A2 SEL1L BCAM DNAJB9 GGCT NUPR1 SLIT1 BET1 DNAJC3 GMPPB PDIA4 TM4SF1 CASP4 DUOX2 HSP90B1
PRIMA1 TMEM140 CDH1 DUSP5 HSPA5 PRSS8 TMEM66 COQ10B DYNLRB2 HYOU1 RIL ZFAND2A CRELD2 ELOVL2 IGF2BP2 RIOK3 Table 3: Target genes of the transcription factor PAX8 (gene symbol) .
miR-223 affects different target genes at multiple cancers like Artemin (oesophageal carcinoma) (41), C/EBP[beta] (leukaemia) (42), E2F1 (leukaemia)(35), EPB41L3 (gastric cancer) (36), Fbxw7/Cdc4 (leukaemia, gastric cancer, oesophageal squamous cell carcinoma) (43,44), FOXOl (colorectal cancer cells) (45), HSP90B1
(osteosarcoma) (46), IGF1R (HeLa, leukemia and hepatoma cells) (37,47), SEPT6 (prostate cancer) (48), LM02 (Leukaemia/lymphoma) (42) and NFI-A (Leukaemia/lymphoma)(49).
This includes a list of 7 well-connected proteins HYOU1, GANAB, CALR, HSPA5, HSP90B1, VCP, and HSPA8 as determined by PPI analysis (Figure 1(c)).
(c) Proteins involved in protein processing in the endoplasmic reticulum proteins are highlighted in red (HYOU1, GANAB, CALR, HSPA5, HSP90B1, VCP, and HSPA8) in the main PPI network.
Szatmary et al., "Upregulation of heat shock proteins (HSPA12A, HSP90B1
, HSPA4, HSPA5 and HSPA6) in tumour tissues is associated with poor outcomes from HBV-related early-stage hepatocellular carcinoma," International Journal of Medical Sciences, vol.
In Figure 3, our results reveal that ADRM1 regulates KPNA2, which promotes proliferation, and is mediated by the aging-related proteins, HSP90B1, CALR, HSPA5, PDIA3, RPN1, and ECT2, the smoking-related proteins, HUWE1, HSPA5, and ECT2, and the epigenetic regulation of ENO1, HSP90B1, CALR, and PDIA3, through the SUP and ER signaling pathways.
The receptor ADRM1 signal triggers the signaling cascade from the smoking-related protein HUWE1 to the aging-related proteins HSP90B1 and RPS20 and the smoking-related TF COPS5.
These authors (76) also identified genes encoding gp96 (adenotin, GRP94; HSP90B1
), LRP (lung-related resistance protein; MVP), galectin-3 binding protein (LGALS3BP), and Mr 67000 laminin receptor (RPSA), although RPSA was not expressed on tumor cells, but on infiltrating vessels.
Selected miRNAs targeted many DEGs that sustained the inflammatory response typically associated with BD including TNF, IL1A, IL10, IL6R, CXCL2, CXCR4, TNFAIP3, OLR1, S100A8, HSP90B1
, and CCL3 (see Figure 3(b) and Table 3).