Analysis of protein-protein interaction: STRING prediction indicated that UTY interacts with heat shock protein 90 kDa alpha, class A member 1 (HSP90AA1
); heat shock protein 90 kDa alpha, class B member 1 (HSP90AB1); ubiquitin specific peptidase 9, Y-linked (USP9Y); lysine (K)-specific demethylase 5D (KDM5D); histone deacetylase 2 (HDAC2); histone deacetylase 1 (HDAC1); histone deacetylase 3 (HDAC3); SET domain containing 2 (SETD2); DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y) and sex determining region Y (SRY) (Figure 4).
3630 INS Identical protein binding and protease binding 4316 MMP7 Peptidase activity and metallopeptidase activity 3880 KRT19 Structural molecule activity and structural constituent of cytoskeleton 7057 THBS1 Calcium ion binding and heparin binding 3320 HSP90AA1
Poly(A) RNA binding and identical protein binding 1508 CTSB Peptidase activity and cysteine-type peptidase activity 7076 TIMP1 Cytokine activity and protease binding.
Embryos cultured at atmospheric oxygen tension (20% [O.sub.2]) also presented higher relative abundance of HSP90AA1, HSPD1, and MORF4L2 genes related to response to cellular stress and DNA damage repair.
In fact, a higher level of transcripts related to cell stress response and DNA damage repair, such as HSP90AA1, HSPD1, MORF4L2, and SOD2, was evidenced in embryos of the 20% [O.sub.2] group (Figure 7(b)).
There were significant changes in the levels of gene expression in 8 of 82 genes (Table 1); HSP90AA1 was included and upregulated by ATSE significantly.
There were significant changes in the levels of gene expression in 38 of 82 genes (Table 2); HSP90AA1 was included and upregulated by ATSE significantly.
That idea is supported by the downregulation of calnexin (Canx), a molecular chaperone which mediates the proper folding of nascent proteins in ER, coupled with upregulation of BiP and HSP90AA1
which bind to misfolded proteins.
For instance, chrysin interacted not only with the genes directly related to inflammation, such as ALOX5, TLR4, MAPL10, MAPK14, and MAPK2K4, but also with genes indirectly related to inflammation, such as HTR2A, HSP90AA1
, and ADCY5.
The smoking-related protein HSP90AA1
and DNA methylation of ECT2 mediate the progression from stage 1 bladder cancer cells to metastasis in stage 4 bladder cancer.
(a) microRNA miR-516-5p miR-517 * miR-526a miR-525 miR-520a * Number of 349 179 212 340 352 predicted target genes Unique target genes Shared with miR-525 CCR2 FAS BCAP29 TOX ACVR2B CD109 IL6ST CD24 AHSA2 CD1A IL9R CD302 ATRN DNAJC25 IRAK3 CFLAR CD2 FLT1 LILRA2 DNAJC21 CD300LB IL17RE MTDH HSP90AA1
CD46 IRAK1 PAPPA IGFBP1 CD93 LILRB5 TLR2 HSF5 PDCD6IP TNFRSF19 IGF1R SOCS2 TNFSF15 IL10RA TRAF6 MMD2 PPARA TLR7 VSIG4 All the targets were predicted by a bioinformatics tool MirTarget2 using miRDB online database.