Table II.- Primers used for amplification of hsp65
gene (Telenti et al., 1993).
The animals were euthanized 14 days after the last dose and IFN-[gamma] and IL-10 were quantified in spleen cell cultures stimulated with recombinant hsp65
From 35 clinical samples considered true positive by clinical parameters for TBM diagnosis, IS6110 PCR was able to detect 32 of them followed by culture and hsp65
KDa (16 samples) and MPB64 (12 samples).
This may improve the longevity of the antigen derived from Hsp65
in the spleen, thus improving the activation of a protective immune response.
The most rapid and accurate method for identification is gene sequencing: 16s rRNA, rpoB, and hsp65
Excessive T-and B-cell responses to major four peptides of Mycobacterium tuberculosis HSP65
and human counterparts of HSP60 were observed in patients with BD who lived in Europe, Far-Eastern Asia, and Middle East 10, 88-90].
Drancourt, "Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65
, sodA, recA and rpoB gene sequencing," International Journal of Systematic and Evolutionary Microbiology, vol.
leprae 10 kDa (GroES), 18 kDa, (34,35) 35 kDa, (36 -38) 45 kDa, (15,39) hsp65
, (40-42) 70 kDa, (43) and Ag85 (30/31 kDa).
For example, a DNA vaccine coding bacterial HSP65
administered in a post-exposure regimen was effective in reducing the bacterial load in mice infected with M.
Several gene regions have been used as molecular targets for the identification of mycobacterial species, these include hsp65
(29,33,34), rpoB gene (35), dnaJ gene (36), 32 kDa protein gene (37), 16S rRNA gene (38,39), 16S-23S rRNA gene spacer (24-26,28,36), promoter region of 16S rRNA (39) and secA1 (40).