The calibration curves of both for HPLC
method and Extraction-HPLC
method, the linearity ranges were both 0.
HbA1c was measured by D10 Haemoglobin testing system (BIO RAD Laboratories) which is based on cation exchange HPLC
and Roche Immunoturbidimetric method (Roche Hitachi (902) Cobas System).
Seguidamente, se agrego agua grado HPLC
(10 mL), y se agito la suspension con ultrasonidos (15 min).
Recovery experiments: Varying concentrations of NVP (50 & 100 mg), 3TC (25 & 50 mg) and d4T (10 & 20 mg) were added to pre-analysed tablet solutions, and the drug amounts were determined by HPLC
and spectrophotometric methods.
Figure 2 shows the representative HPLC
chromatogram of the serum, measured at the wavelength of 254 nm.
52% that were stored frozen since their use in a Swedish CDT harmonization program in 2002-2003 were reanalyzed by HPLC
in November 2007.
Waters has chosen a proprietary approach that complements the success of its Alliance HPLC
in pharmaceutical and QA/QC applications.
All solvents were HPLC
grade from Burdick and Jackson (Muskegon, MI).
If you read this column you know we (and most analytical chemists) favor HPLC
analyses over all others where it is applicable.
Better still, scientists don't have to lose several hours reconfiguring the HPLC
to run the separate tests for sugars and acids.
The reported HPLC
method for GLP is advantageous due to simplicity, accuracy, specificity, and at the same time has very short run-cycle time.