Knockdown of HOTAIRM1 can partially counteract RA-induced expression of HOXA1 and HOXA4 during the RA-induced granulocyte differentiation of NB4 cells and selectively attenuated induction of CD11b and CD18 expression, two hallmarks for granulocyte differentiation.
HOTAIRM1 regulates RA-induced expression HOTAIRM1 of HOXA1 and HOXA4 during the RA-  induced granulocyte differentiation of NB4 cells and promotes induction of CD11b and CD18 expression, two hallmarks for granulocyte differentiation.
In mice, RAREs are found in Hoxa1, Hoxa4, Hoxb1, Hoxb4, and Hoxd4 [5-14].
Our analysis showed that HOXA4, HOXA9, and HOXD10 are expressed more in colonic SCs than in proliferating cells or differentiating crypt cells .
We previously reported that HOXA4, HOXA9, and HOXD10 are expressed in normal colonic SCs and dysregulation of HOX genes leads to aberrant SC differentiation, contributing to CRC development and growth.
Because we previously found that HOXA4 and HOXD10 are expressed in crypt SCs, we measured HOXA4 and HOXD10 levels in CRC cases (n = 220) based on high versus low expression (cutoff at 25th percentile).
Previously, we identified HOXA4, HOXA9, and HOXD10 signatures for normal colonic SCs and that these HOX genes are upregulated during CRC development.
We have shown that HOXA4 and HOXA9 are upregulated in CRC SCs [49, 50] and that siRNA knockdown of HOXA4 and HOXA9 reduces proliferation and sphere-formation ability of CRC SCs.