Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1.
Perturbation of cranial neural crest migration by the HNK-1 antibody.
The topics include sophisticated modes of sugar recognition by intracellular lectins involved in controlling the quality of glycoproteins, the structure and activities of natural complex polysaccharides, click chemistry in carbohydrate-based drug development, and the functional enzyme complex involved in HNK-1
Monocular enucleation of tadpoles resulted in visibly diminished HNK-1 staining of the stratum opticum in the denervated optic tectum contralateral to the enucleation.
1 showed that the HNK-1 carbohydrate was present on the antigen immunoprecipitated with MAB 1H12 (Fig.
To analyze more closely whether MAB 1H12 is specific for the HNK-1 epitope, a competitive ELISA was performed to measure what effect pre-incubation of frog brain antigen with various reagents had on subsequent recognition by 1H12-HRP conjugate (Table 1).
In the present study, HNK-1 antibody is used to visualize the sequence of ciliary development in Capitella sp.
There is no evidence of HNK-1 reactivity during cleavage or gastrulation.
Some neuronal components of the pharyngeal pads were visualized using indirect immunofluorescence techniques, with the HNK-1 monoclonal antibody (from the American Type Culture Collection) serving as the probe.
The HNK-1 antibody recognizes a specific carbohydrate moiety frequently found on membrane glycoproteins of neural cells (Linser, 1991; Bakker et al.
Sections of a pharyngeal pad immunostained with the HNK-1 antibody are shown in Figure 4D-F.