Three parameters, grain yield (t ha-1), test weight (kg hl-1) and 1000-grain weight (g) were analysed.
Hectoliter weight: During the first year, achieved the highest test weight at NP1K variant (67.72 kg hl-1), while the lowest test weight was the NK treatment (63.56 kg hl-1).
On heating the complexes [M[(HL).sub.2] x n[H.sub.2]O (M = [Co.sup.II], or [Ni.sup.II], and HL = HL-1 or HL-2) in air oven between 60 and 80[degrees]C for one hour, is loss [H.sub.2]O and loss in weight incurred in complexes correspond to loss of required for 2[H.sub.2]O, and no change in colour of the complexes that was noticed which indicated that [H.sub.2]O molecules are not coordinated to metal atom rather they are held up in crystal lattices.
The v(C = S) of ligand shifts to 760-780 [cm.sup.-1] in complexes [M(HL-1)2] and [M(L-1)B] type of complexes indicating the coordination of thioamide (C = S) through deprotonated thiol sulphur.
MSC express connexins and are able to form gap junctions with cardiac ventricular myocytes  and HL-1 cardiac cells .
In the current study, we examined the interaction of MSC with cardiac HL-1 cells in a coculture system.
Cardiac HL-1 cells were generously supplied by W.C.
MSC or HL-1 monolayers were labeled with various fluorescent dyes prior to coculture.
The murine atrial cardiomyocyte cell line HL-1 (18),a generous gift from W.C.
We used the plasmids to transfect HEK 293 and HL-1 cells using FuGENE reagent (Roche Diagnostics) or Lipofectamine 2000 (Invitrogen) according to manufacturers' instructions.
Briefly, the conditioned medium containing pro-ANP, pro-BNP, and pro-CNP and their derivatives was collected from cultured HEK 293 and HL-1 cells and concentrated with an ultracentrifugal filtration device (Millipore).
Comet Automation Systems has introduced two new pieces of auxiliary equipment: the HL-1
Vacuum Hopper Loader, and the HL-2 Self-Contained Vacuum Hopper Loader.