AMBP

(redirected from HI30)

AMBP

A gene on chromosome 9q32-q33 that encodes alpha-1-microglobulin/bikunin precursor protein, which is expressed in liver and secreted in plasma. Alpha-1-microglobulin belongs to the superfamily of lipocalin transport proteins and plays a role in regulating inflammation; bikunin belongs to the superfamily of Kunitz-type protease inhibitors and plays a key role in many physiological and pathological processes, by inhibiting trypsin, plasmin and lysosomal granulocytic elastase, as well as calcium oxalate crystallisation.
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Antibody panel for immunophenotyping Antibody Fluorochronie Clone Company Anti-CD9 FITC M-L13 BD Bioscience Anti-CD10 PE HI 10a BD Bioscience Anri-CD29 PE MAR4 BD Bioscience Anti-CD34 FITC 581 BD Bioscience Anti-CD38 FITC HIT2 BD Bioscience Anti-CD44 PE 515 BD Bioscience Anti-CD45 PE HI30 BD Bioscience Anri-CD73 PE AD2 BD Bioscience Anti-CD105 PE 166707 R&D systems Anti-CD133 PE W6B3C1 BD Bioscience Anti-Nanog - Polyclonal Abeam Anti-Oct-4 - Polyclonal Abeam Anti-SSEA-4 - MC 813-70 BD Bioscience Anri-Stro-1 - STRO-1 R&D systems Anti-rabbit Ig FITC Polyclonal Abeam Anti-mouse IgG FITC Polyclonal Sina Biotech Table 2.
Marker Fluorochrome Host Clone CD14 PE Mouse anti-human M[phi]P9 CD16 FITC Mouse anti-human B73.1 CD34 FITC Mouse anti-human 581 CD45 PE Mouse anti-human HI30 CD133 APC Mouse anti-human AC133 CD235a FITC Mouse anti-human GA-R2 (HIR2) CD309 PE Mouse anti-human 89,106 Marker Manufacturer CD14 Becton Dickinson Bioscience CD16 Becton Dickinson Bioscience CD34 Becton Dickinson Bioscience CD45 Becton Dickinson Bioscience CD133 Miltenyi Biotec CD235a Becton Dickinson Bioscience CD309 Becton Dickinson Bioscience PE: phycoerythrin; FITC: fluorescein isothiocyanate; APC: allophycocyanin.
The following antibodies for flow cytometry were used: anti-CD107a-FITC (H4A3), anti-[CD3.sup.-]Alexa700 (UCHT1), anti-CD56-PE-Cy7 (B159), and anti-CD45 (HI30), purchased from BD Biosciences; anti-ULBP1-PE (170818), anti-ULBP2/5/6-PE (165903), anti-ULBP3-PE (166510), anti-MICA (159227), anti-MICB (236511), antiTRAIL/R2-APC (17908), anti-CD155/PVR-PE (300907), and anti-Nectin-2/CD112-APC (610603), purchased from R&D Systems; W6/32 which recognizes human fully assembled MHC class I heavy chains; and goat F(ab')2 Fragment anti-mouse IgG FITC (IM1619, Dako) for flow cytometry.