For the HI test
, we treated the samples with receptor-destroying enzyme (RDEII; Denka Seiken, http://www.keyscientific.com) at 37[degrees]C for 16 h, followed by heat inactivation at 56[degrees]C for 30 min.
Humoral immune response to rHVT-H5 vaccination was tested by HI test
against HA antigens homologous either with the vaccine virus insert or with the challenge virus.
(11) The test was considered positive when the HA end-point titre was depressed by three or more dilutions in the HI test
. A titre of 1/16 was considered positive.
Statistical analysis: The data regarding HI test
was processed for calculating geometric mean titer (GMT) on 21, 28 and 35 days post-vaccination (DPV).
For confirmation of vaccine virus as APMV-1, HI test
was performed using known positive antisera of APMV-1.
Map of Harris County and the City of Houston, Texas, showing the geographical distribution of HI test
results for WNV, SLE, EEE, and WEE in 2015.
The virus Neutralization test (NT) was far more sensitive in comparison to HI test
Antibody titer for NDV was determined from each serum sample using the OIE HI test
The positive results showed that 81.7 % of ELISA positive cases had titer [greater than or equal to] 32 for H9N2 influenza in HI test
. Although positive result were found more in dogs with general or respiratory signs, no significant differences were observed in the evaluated factors and seropositivity.
The HA agent was confirmed as AI (H9 type) by using HI test
with AIV-H9 specific antisera (Muhammad et al., 2001).
Virus titer calculated by HI test
ranged from 1:64 to 1:512.
As a companion to the Hydraulic Institute Standards, together with the HI Test
Standards, the Engineering Data Book completes the data for solving problems related to the pumping and transfer of fluids.