And K13 mRNA expression was inhibited in a time-dependent manner, whereas Hes1
mRNA expression was decreased in concentration- and time-dependent manners.
C, The protein levels of Hes1
, N-cadherin, E-cadherin in tumor cells were examined with Western blot.
The data showing TCDD-induced alterations of Notch and Hes1
expression combined with the shift in the proportion of cells in c-[Kit.sup.+]DC[F.sup.int] to c-[Kit.sup.+]DC[F.sup.hi] are suggestive of premature maturation of the hematopoietic stem cell pool, which effectively depletes the number of l ong-t erm hematopoietic stem cells.
Normal mice were subjected to burn injury, and the protein expression of Notch1 and Hes1
in myocardium tissue was examined at various time points from 0 to 24 h.
The micro-RNA 199b-5p regulatory circuit involves Hes1
, CD15, and epigenetic modifications in medulloblastoma.
Reverse Transcription and qPCR Assay for GATA4, cTnI, Notch1, and Hes1
After fixation with cold acetone for 10 min, the tissues were incubated with 10% goat serum containing 0.1% Triton X-100 and 1% BSA (room temperature (RT); 1h) for Cytokeratin 19 (CK19) and with 1% BSA and goat serum (RT; 30 min) for hairy enhancer of split 1 (Hes1
(29) The tissue microarrays with densities of 56 spots/[cm.sup.2] were sectioned to 5-[micro]m thickness for immunohistochemical profiling of Notch1, Notch2, and Hes1
expression and intracellular distribution, respectively.
Gene Microarray Q-PCR PLK2 2.2 [+ or -] 0.1 1.8 [+ or -] 0.4 SGK 3.9 [+ or -] 0.2 3.4 [+ or -] 1.5 SE20-4 2.5 [+ or -] 0.2 2.4 [+ or -] 0.2 BHLHB2 2.8 [+ or -] 0.1 4.2 [+ or -] 0.2 CCNL1 3.1 [+ or -] 0.2 2.6 [+ or -] 0.4 C8FW 4.0 [+ or -] 0.2 2.3 [+ or -] 0.2 HES1
8.9 [+ or -] 0.9 1.7 [+ or -] 0.4 FOSB 52 [+ or -] 1.8 2.6 [+ or -] 0.1 DUSP2 17 [+ or -] 0.0 1.7 [+ or -] 0.1 PPP1R15A 4.6 [+ or -] 0.1 0.9 [+ or -] 0.2 NFKB1A 3.7 [+ or -] 0.3 0.9 [+ or -] 0.2 NEU1 2.2 [+ or -] 0.1 1.1 [+ or -] 0.1 DDAH1 -2.2 [+ or -] 0.0 -1.8 [+ or -] 0.1 NFATC1 -2.9 [+ or -] 0.0 -1.8 [+ or -] 0.2 NVKB1 -2.8 [+ or -] 0.0 -5.2 [+ or -] 0.8 Data are mean [+ or -] SD of three independent measurements, which represent fold change on [log.sub.2] scale compared with data obtained from control group.
They revealed that EVs from MSCs contain mRNAs involved in transcription (e.g., MDFIC, POU3F1), angiogenesis (e.g., HES1
, TCF4), adipogenesis (e.g., CEBPA, KLF7), and transforming growth factor- (TGF-) [beta] signaling (e.g., TGFB1, TGFB3).
Ishikawa, "Human Sir2-related protein SIRT1 associates with the bHLH repressors HES1
and HEY2 and is involved in HES1- and HEY2-mediated transcriptional repression," Biochemical and Biophysical Research Communications, vol.