HEPES


Also found in: Dictionary, Thesaurus, Acronyms, Idioms, Encyclopedia, Wikipedia.

HEPES

4-(2-Hydroxyethyl)-1-piperazine ethanesulfonic acid; a compound lacking in pharmacologic effects and widely used as a biologic buffer in in vitro experiments.
References in periodicals archive ?
Although [delta]PAP in the HEPES 2 group tended to be higher during the acute and sustained phases of HPV, it was only significant during min 2-4 compared with the control HOX group.
3]H]adenosine was performed in a final assay volume of 300 [micro]L of 20 mmol/L Tris-40 mmol/L HEPES, pH 7.
3], 20 mM HEPES, 50 [micro]g/L gentamycin, 1 uM insulin, 10 [micro]M hydrocortisone, 2% Ultroser SF and 2 mg/L of the protease inhibitor aprotinin (Fluka, Buchs, Switzerland).
The size of this signal decreased as the concentration of the pH buffer HEPES was increased, consistent with the hypothesis that the signal detected indeed reflected hydrogen ions.
0 g/L bovine albumin (calcium free) in 100 mmol/L HEPES buffer (pH 7.
To 5 [micro]L of purified proCPU (from different batches with a corresponding CPU activity of 900-2650 U/L), 10 [micro]L of thrombin-thrombomodulin in 20 mmol/L HEPES, 5 mmol/L [CaCl.
265 mmol/L) dobutamine was added to HEPES buffer in the presence of various concentrations of horseradish peroxidase.
HEPES, fibrinogen, Arg-Gly-Asp-Ser (RGDS) peptide, bovine serum albumin (V-BSA), glucose, wortmannin, ADP, and U46619 were obtained from Sigma (St.
The leftmost portion of the trace indicates the size of the differential signal recorded from one cell bathed in a skate-modified Ringer containing 2 mM of the pH buffer HEPES and adjusted to pH 7.