HEPES


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HEPES

4-(2-Hydroxyethyl)-1-piperazine ethanesulfonic acid; a compound lacking in pharmacologic effects and widely used as a biologic buffer in in vitro experiments.
References in periodicals archive ?
d] values were calculated by fitting free bilirubin titration data measured in HEPES buffer at pH 7.
The ACE catalytic activity is influenced by the nature of the substrate; Hip-Gly-Gly shows the highest activity on a HEPES medium, at pH 8.
1 (C57) (Tsai 1996) mouse mast cells were cultured in DMEM supplemented with 10% heat-inactivated FCS, 4 mM L-glutamine, 25 mM HEPES, 50 [micro]M 2-mercaptoethanol, and 100 [micro]g/mL penicillin/streptomycin (complete DMEM).
1) at 37[degrees]C, by addition of 40 [micro]L of 1200 mmol/L HEPES acid (Fisher Biotech) per milliliter of serum.
1 mM NEAA and 1 mM sodium pyruvate were added to the DMEM medium and 1 mM sodium pyruvate and 10 mM HEPES were added to the RPMI 1640 medium.
1) at 37 [degrees]C, before ultrafiltration, by adding 40 [micro]L of 1200 mmol/L HEPES acid (Fisher Biotech) per mL serum.
In separate experiments, cell samples were collected 72 hr after exposure either for GSH-R activity measurements or for culture in HEPES for 16 hr with or without cystine (24 [micro]g/mL) for CYSH and GSH assessment.
We dissolved shellfish extracts in 2 mL acetone (100%), of which 1 mL was dried down and resuspended in a HEPES binding buffer (50 mM, pH 7.
4] in HEPES buffer and for 4-5 h at 500g for serum samples.
We chose HEPES, which is available worldwide in the highest quality, but 2-(N-morpholino)propanesulfonic acid or N,N'-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid may be used as well.