HEPES


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HEPES

4-(2-Hydroxyethyl)-1-piperazine ethanesulfonic acid; a compound lacking in pharmacologic effects and widely used as a biologic buffer in in vitro experiments.
Farlex Partner Medical Dictionary © Farlex 2012
References in periodicals archive ?
After analyzing the impact of HEPES and hydroxylamine on the final tips shape, we estimated that the optimal molar ratio of hydroxylamine :Au ions is 3 : 1, where this amount is enough to avoid the intervention of HEPES in the reduction.
However, no significant alteration was observed between the maximum values of [delta]PAP in all experimental groups during short term hypoxic gas ventilation even though PAP tended to be lower with more variation in the HEPES 1 group compared to others (data are not shown).
The devices were centrifuged for 2 h at 500g for [T.sub.4] in HEPES buffer and for 4-5 h at 500g for serum samples.
AM from the BALF of each rat were suspended in Eagle Minimum Essential Medium culture medium (Sigma) containing 1 mM glutamine, 100 [micro]g/mL streptomycin, 100 units/mL penicillin, 10% heat-inactivated fetal bovine serum, and 10 mM HEPES. We added aliquots of 1 mL cell suspensions from each rat, adjusted to 1 x [10.sup.6] AM, to each well of a 12-well tissue culture plate.
Initially, we used 10 mM Hepes-KOH, pH 7.4 (Hepes) for the starvation medium because we recently, found that long-term starved cells survive better in Hepes than in 10 mM Tris-HCl, pH 7.4 (Tris) (Hellung-Larsen et al., 1993).
Culture conditions included the use of phenol red-free DMEM/F12 medium (Sigma) supplements with 14.3 mM NaHC[O.sub.3], 20 mM HEPES, 50 [micro]g/L gentamycin, 1 uM insulin, 10 [micro]M hydrocortisone, 2% Ultroser SF and 2 mg/L of the protease inhibitor aprotinin (Fluka, Buchs, Switzerland).
The platelets were then washed twice in Tyrode's buffer (137 mM NaCl, 2.68 mM KCl, 0.42 mM Na[H.sub.2][PO.sub.4], 1.7 mM Mg[Cl.sub.2]) containing 10 mM HEPES (pH 6.5) and resuspended in Tyrode's buffer containing 0.2% BSA, 0.1% glucose, and 10 mM HEPES (pH 7.35).
After chemical treatments, cells were washed with ice-cold PBS/EDTA, and then 1 mL of ice-cold lysis buffer (50 mM HEPES, pH 7.2, 150 mM NaCl, 0.2% NP-40, 2 mM EGTA, 2 mM EDTA, and protease inhibitors) was added to each flask.
After preincubation for 2 hr in 10 mL complete Williams' medium E (supplemented with 2 mM L-glutamine, 0.02 IU insulin/mL, and 10% FBS), we washed the flasks with prewarmed Hepes buffer (pH 7.4) to remove the unattached dead cells.