HEPES


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HEPES

4-(2-Hydroxyethyl)-1-piperazine ethanesulfonic acid; a compound lacking in pharmacologic effects and widely used as a biologic buffer in in vitro experiments.
References in periodicals archive ?
After analyzing the impact of HEPES and hydroxylamine on the final tips shape, we estimated that the optimal molar ratio of hydroxylamine :Au ions is 3 : 1, where this amount is enough to avoid the intervention of HEPES in the reduction.
However, no significant alteration was observed between the maximum values of [delta]PAP in all experimental groups during short term hypoxic gas ventilation even though PAP tended to be lower with more variation in the HEPES 1 group compared to others (data are not shown).
The devices were centrifuged for 2 h at 500g for [T.sub.4] in HEPES buffer and for 4-5 h at 500g for serum samples.
AM from the BALF of each rat were suspended in Eagle Minimum Essential Medium culture medium (Sigma) containing 1 mM glutamine, 100 [micro]g/mL streptomycin, 100 units/mL penicillin, 10% heat-inactivated fetal bovine serum, and 10 mM HEPES. We added aliquots of 1 mL cell suspensions from each rat, adjusted to 1 x [10.sup.6] AM, to each well of a 12-well tissue culture plate.
Initially, we used 10 mM Hepes-KOH, pH 7.4 (Hepes) for the starvation medium because we recently, found that long-term starved cells survive better in Hepes than in 10 mM Tris-HCl, pH 7.4 (Tris) (Hellung-Larsen et al., 1993).
Culture conditions included the use of phenol red-free DMEM/F12 medium (Sigma) supplements with 14.3 mM NaHC[O.sub.3], 20 mM HEPES, 50 [micro]g/L gentamycin, 1 uM insulin, 10 [micro]M hydrocortisone, 2% Ultroser SF and 2 mg/L of the protease inhibitor aprotinin (Fluka, Buchs, Switzerland).
The platelets were then washed twice in Tyrode's buffer (137 mM NaCl, 2.68 mM KCl, 0.42 mM Na[H.sub.2][PO.sub.4], 1.7 mM Mg[Cl.sub.2]) containing 10 mM HEPES (pH 6.5) and resuspended in Tyrode's buffer containing 0.2% BSA, 0.1% glucose, and 10 mM HEPES (pH 7.35).
After chemical treatments, cells were washed with ice-cold PBS/EDTA, and then 1 mL of ice-cold lysis buffer (50 mM HEPES, pH 7.2, 150 mM NaCl, 0.2% NP-40, 2 mM EGTA, 2 mM EDTA, and protease inhibitors) was added to each flask.
After preincubation for 2 hr in 10 mL complete Williams' medium E (supplemented with 2 mM L-glutamine, 0.02 IU insulin/mL, and 10% FBS), we washed the flasks with prewarmed Hepes buffer (pH 7.4) to remove the unattached dead cells.
Tenders invited for Grease, paraffin oil, hepes, tris buffer
Tenders are invited for Supply Of Higlutaxl Rpmi-1640 W/L-Alanyl-L-Glutamine, Hepes Buffer And Sodium Bicarbonate 1x Liquid Culture Medium 18 Bottlesx500ml
RBC is offering cGMP HEPES, MOPS, and MES in convenient prepackaged development quantities on their new Catalog site.