EPHA3

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EPHA3

A gene on chromosome 3p11.2 that encodes a member of the ephrin-A receptor subfamily of receptor tyrosine kinases, which promiscuously bind membrane-bound ephrin-A family ligands residing on adajcent cells, leading to contact-dependent bidirectional signalling into neighbouring cells. EPHA3 mediates developmental events, especially in the nervous system—once activated by EFNA5, it regulates cell–cell adhesion, cytoskeletal organisation and cell migration. It plays a role in cardiac cell migration and differentiation, and it regulates formation of the atrioventricular canal and septum during development. It is involved in the retinotectal mapping of neurons and may control the segregation—but not the guidance—of motor and sensory axons during neuromuscular circuit development.
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We cultured HEK 293 cells in Dulbecco modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS).
We used the plasmids to transfect HEK 293 and HL-1 cells using FuGENE reagent (Roche Diagnostics) or Lipofectamine 2000 (Invitrogen) according to manufacturers' instructions.
Briefly, the conditioned medium containing pro-ANP, pro-BNP, and pro-CNP and their derivatives was collected from cultured HEK 293 and HL-1 cells and concentrated with an ultracentrifugal filtration device (Millipore).
HEK 293 and HL-1 cells expressing pro-ANP, pro-BNP, and pro-CNP were grown in 6-well plates.
Whilst the principal goal of the HEK project will continue to focus on searching for moons, this first planetary system discovered by HEK demonstrates the unexpected discoveries possible with transit analysis," said Kipping.
HEK 293 cells were treated with a furin inhibitor, decanoyl-Arg-Val-Lys-Arg-chloromethyl ketone (DecRVKR-CMK) (Enzo Life Sciences), or cotransfected with a furin-specific small interfering RNA (siRNA) (Santa Cruz Biotechnology) along with proBNP transfection.
We separated proteins from the conditioned media of HEK 293 cells transfected with the plasmid expressing proBNP-T71A by size-exclusion chromatography using a Superdex 75 10/300 GL column (GE Healthcare) as described (10).
HEK 293 cells transiently transfected with the corin-expressing plasmid were used for the experiments on corin-mediated cleavage of proBNP.
HEK 293 cells that normally express functional furin (13,23) were transfected with the plasmids encoding proBNP-T71A or proBNP-WT and treated with a furin inhibitor, Dec-RVKR-CMK, or cotransfected with a furin-specific siRNA.
The HEK tool enables us to characterize significant features and events that will be of interest to solar physicists, and make these data subsets easily accessible.
The HEK is designed to catalog interesting solar events and features and to present them to members of the solar physics community in such a way that guides them to the most relevant data for their purposes.
The HEK was developed as an integrated metadata system designed with the following goals in mind: