HDAC1 and HDAC2
integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130.
Preceded by an initial denaturation of 3 min at 95[degrees]C, and followed by a continuous melt curve from 65-95[degrees]C, the PCR conditions for mice samples were 39 cycles of 30 s at 95[degrees]C, 10 s at 58[degrees]C for Hdac1 / Hdac2
/ Hdac3 / Vangl2 / Scrib / Rac1 / Gapdh ; for H9C2 samples were 39 cycles of 30 s at 95[degrees]C, 10 s at 55[degrees]C for Hdac1/Hdac2/Hdac3/Vangl2/Scrib/Rac1/Gapdh .
MeCP2 has also its role in anchoring a multiprotein repressory complex to the DNA which is responsible for deacetylation of histones and alterations of chromatin by recruiting deactylases HDAC1 and HDAC2
also shown in Fig.
Compared with control mice, basal mRNA levels of DNA (cytosine-5)-methyltransferases 1 (Dnmt1), 3A (Dnmt3a), and 3B (Dnmt3b) and histone deacetylases 1 (Hdac1) and 2 (Hdac2
) were lower in adult ADX/OVX mice exposed to genistein as neonates (Figure 9A).
For example, HDAC2
is highly expressed in tumours and related to p16; by inhibiting HDAC2
, p16 activity is promoted and cells are arrested in G1/S .
Giacometti et al., "HDAC2
negatively regulates memory formation and synaptic plasticity," Nature, vol.
In the present study, the association between the activation of PAR4 and expression of p16 protein and gene, as well as the enrichments of DNMT1 and HDAC2
on the p16 promoter, was examined by Western blotting, quantitative real-time PCR (qRT-PCR), and chromatin immunoprecipitation-PCR (ChIP-PCR) methods to identify the potentially diagnostic or therapeutic value of PAR4 in ESCC.
However, BHB appears to inhibit HDAC2
in microvascular and neuronal brain cells , and BHB-induced HDAC inhibition is thought to have a role in the antiseizure effects of ketogenic diets .
Park et al., "Cancer/testis antigen CAGE exerts negative regulation on p53 expression through HDAC2
and confers resistance to anti-cancer drugs," The Journal of Biological Chemistry, vol.
Gauthier et al., "Role of Brg1 and HDAC2
in GR trans-repression of the pituitary POMC gene and misexpression in Cushing disease," Genes & Development, vol.
Moreover, this is supported by a recent study indicating that the expression of nuclear HDAC1, HDAC2
, and HDAC3 proteins was increased in carcinomas compared with benign tumors .
The authors reported that HDAC1 and HDAC2
were downregulated, which are associated with an increase in H3K4me3 and H3K9Ac and which are associated with an active chromatin state .