Damage of Escherichia coli membrane by bactericidal agent polyhexamethylene guanidine hydrochloride: micrographic evidences.
Antifungal activity of the cationic antimicrobial polymer-polyhexamethylene guanidine hydrochloride and its mode of action.
The resin TGF was synthesized by the condensation of thymol and guanidine hydrochloride
with formaldehyde, in the presence of an acidic catalyst using microwave irradiation.
Louis, Missouri), guanidine hydrochloride (Acros Organics, Fair Lawn, New Jersey), and lithium perchlorate (Alfa Products, Beverly, Massachusetts).
The chaotropic modifiers urea, guanidine hydrochloride, dicyandiamide, and lithium perchlorate and the surfactants SDS and CTAB were added to soy flour adhesives.
mol/L [+ or -] SDJ Natural isolates Scrapie ES/8/10/2 2.19 [+ or -] 0.18 CH1641-like 99-454 2.00 [+ or -] 0.06 99-321 2.41 [+ or -] 0.49 TR316211 2.82 [+ or -] 0.08 Experimental samples CH1641 241/74 2.07 [+ or -] 0.05 Sheep BSE 301/16 ([section]) >4 301/44 ([section]) >4 302/90 ([paragraph]) 3.8; >4; >4 * PrP, prion protein; GdnHC[L.sub.1/2], guanidine hydrochloride
at a concentration able to dissolve half the insoluble aggregates in a brain homogenate; BSE, bovine spongiform encephalopathy.
The eluate from the column was mixed with a reagent consisting of 50 mmol/L guanidine hydrochloride
, 0.5 mmol/L sodium metaperiodate, and 0.1 mol/L boric acid in 200 mL/L acetonitrile and adjusted to pH 11.0 with 5 mol/L NaOH.
The highly active particulate matter obtained from the ovary was partially solubilized and extracted with a buffer containing 2 M guanidine hydrochloride, then purified.
Furthermore, the dilution of a solution containing high concentrations of ammonium sulfate always causes a marked decrease in the activity, whereas the addition of 1-2 M guanidine hydrochloride or 0.01% cationic detergent (such as LCC or hexadecyltrimethylammonium bromide) to a luciferase solution often results in an increase in activity.
A silica spin disc filter unit or a spin filter containing silica matrix suspended in 0.75 mol/L potassium acetate (pH 4.6) containing 4 mol/L guanidine hydrochloride
(50 [micro]L) was placed in a 1.5-mL microcentrifuge tube, and the plasmid DNA-containing solution was applied to the spin filter units.
The precipitate was mixed with 5 ml of 10 mM Tris-HCl buffer, pH 7.7, containing 2 M guanidine hydrochloride
, 1 M KCl and 1% glucose, then sonicated briefly.