Aiming to search for accurate methods to identify strains showing atypical results in the coagulation assays, we estimated the identification potential of four different techniques: Matrix-Assisted Laser Desorption Ionization-Times of Flight Mass Spectometry (MALDI-TOF), PCR-restriction fragment length polymorphism (PCR-RFLP) of the chaperonine gene groEL
, and DNA sequencing of the 16S rRNA and elongation factor Tu gene tuf
DNA'lardan L.acidophilus ve L.casei miktarlarinin saptanmasi icin tablo 1'deki Hsp60, groEL
bolgesi spesifik primer ve problar kullanilarak LightCycler 480 II (Roche Diagnostics, Mannheim, Almanya) real-time PCR sisteminde, Fast Start Essential Probe Master Mix kiti (Roche Diagnostics GmBH, Mannheim Almanya) ile her bir reaksiyona tablo 2'deki reaktif miktarlari yuklenerek uretici direktifleri dogrultusunda calisma yapildi (11, 12).
Versatile roles of the chaperonin GroEL
in microorganism-insect interactions.
, DnaK, GroES, Tig, DnaJ and GrpE are major cytoplasmic chaperones in E.
This increased thermal tolerance is partially due to the activation of conserved heat shock proteins (Hsps) (e.g., DnaK and GroEL
) under sublethal heat temperatures (Doyle et al., 2001; Ferreira et al., 2001; Hill et al., 2002).
We confirmed EMLA positivity of the samples by amplifying and sequencing the EMLA citrate synthase (gltA) and heat shock protein (groEL
) genes (3).
A highly sensitive quantitative real-time PCR assay based on the groEL
gene of contemporary Thai strains of Orientia tsutsugamushi.
Roseman et al., "ATP-bound states of GroEL
captured by cryo-electron microscopy," Cell, vol.
The synthesis of DnaK and GroEL
was induced during infection, evidenced by antibodies detected in sera from patients with S.
CpaB, CpaF/TadA, TadB, pilQ/Cpac, pilV, flp1, flp2 (genes associated with type IV pilus secretion), chemotaxis protein (MCP: methyl accepting chemotaxis protein, chemotaxis protein CheY), heat-shock protein (GroES and GroEL
), cell wall-associated protein (wapA), host interaction (hit) locus orf.
Identification of Salmonella isolated from poultry by MPCR technique and evaluation of their hsp groEL
gene diversity based on the PCR-RFLP analysis.