Following conjugation of [CD8.sup.+] T cells with an appropriate target cell, lytic granules are transported to the point of contact with the target, and granule perforin and granzyme
B are released into the immune synapse between [CD8.sup.+] T cells and the target.
Paired box protein PAX5, CD1a, terminal deoxynucleotidyl transferase, CD117, granzyme
B, and CD34 were all negative in the neoplastic cells, ruling out B-cell involvement or acute lymphoid or myeloid leukemia.
Fuchs and colleagues described an ILCs1-like subset, characterized by the expression of NKp44, NKp46, CD56, CD103, granzyme
, and perforin and located in the intestinal epithelial compartment (intraepithelial ILC1-like cells).
The antibodies and experimental conditions used to detect the expression of EZH2, CD3, CD8, CD4, Tbet, granzyme
B (GZMB), Foxp3, PD1, PDL1, CD56, CD20, HLA class I, CD14, CD68, CD163, CD209, CD21, and VEGFR2 are shown in Supplementary Table S1.
For instance, results are regularly negative for CD3, CD5, cytotoxic molecules (granzyme
B1, TIA1, and perforin), CD20, CD79a, PAX5, myeloperoxidase (MPO), CD117, and lysozyme.
Tak, "Analysis of the cell infiltrate and expression of matrix metalloproteinases and granzyme
B in paired synovial biopsy specimens from the cartilage-pannus junction in patients with RA," Annals of the Rheumatic Diseases, vol.
Antibodies to FasL, Fas, Hsp70, and Tag7 inhibit the cytotoxic activity of the CD4+CD25+CD127+ subpopulation, while antibodies to granzyme
B have no effect, although we have detected granzymes
inside of lymphocytes via intracellular antibody staining (Supplemental Figure 8).
Fehniger et al., "Granzyme
B and perforin are important for regulatory T cell-mediated suppression of tumor clearance," Immunity, vol.
Using immunohistochemical studies, the neoplastic cells are [CD3.sup.+], [CD5.sup.-], [CD7.sup.+], [CD8.sup.+/-], [CD4.sup.-], [CD56.sup.-], and TCR[[beta].sup.+/-], and are positive for such cytotoxic markers as TIA-1, granzyme
, and perforin (Figure 3).
These include tumor necrosis factor- (TNF-) [alpha] [10, 11], Fas/Fas ligand [12-14], perforin/ granzyme
B [15-17], and granulysin .
Tostes Jr., "Characterization of inflammatory infiltrates in chronic chagasic myocardial lesions: presence of tumor necrosis factor-alpha+ cells and dominance of granzyme
A+, CD8+ lymphocytes," The American Journal of Tropical Medicine and Hygiene, vol.
Subcutaneous panniculitis-like T-cell lymphoma has a mature [alpha][beta] cytotoxic T-cell phenotype, and is usually [CD3.sup.+], [CD8.sup.+], [CD4.sup.-], with expression of cytotoxic markers such as TIA1, perforin, and granzyme
B (Figure 3, C and D).