Sections measuring 5 pm width were obtained and stained using hematoxylin and eosin (HE), Gomori
's trichrome or Von Kossa stain to observe the tissue morphology.
The sarcoplasmic rods were seen as small, clustered dark eosinophilic sarcoplasmic inclusions on hematoxylin and eosin stain and were red on Gomori
Standard in-office potassium hydroxide (KOH) testing can't distinguish one species of fungus from another, nor can pathology with Gomori
methenamine silver (GMS) or Periodic acid-Schiff (PAS) staining.
"Older generations look to millennials for how to incorporate the tech into the jobsite," said Paul Gomori
, application engineering manager, JCA Electronics.
Special stains for fungal organisms, including Gomori
methenamine silver (GMS) and periodic acid-Schiff (PAS) were strongly positive (figure 3).
The hyphae and conidiospores were Gomori
methenamine silver (GMS) positive.
Although Warthin-Starry and Gomori
trichrome stains have been optimal for light microscopy, the spores can be confused with yeast cells because of their appearance (2,3; Figure 1).
Histochemical studies such as PAS, Gomori
methenamine silver (GMS) and acid fast could be used to detect microorganism.
They can be visualized on light microscopy by the Gomori
trichrome stain, appearing as dark blue structures localized in the sarcoplasm, predominantly in regions with disrupted sarcomere structure.
Tissue sections of 5-8 mm thickness were cut and utilized for utilized for various histological and histochemical staining methods like Haematoxylin Eosin (H&E) method, Masson's trichrome method, Verhoeff's Elastic stain, Periodic Acid-Schiff reaction for neutral mucopolysaccharides, Sudan Black B for fats (Singh & Sulochana, 1996) and Gomori
's method for reticular fibres (Spencer & Bancroft).
Other special stains done routinely include Gomori
methenamine silver (GMS), Periodic acid-Schiff (PAS), Gridley's method, Mucicarmine and Masson-Fontana (for pigmented fungi).