ProtoKinetix is a molecular biotechnology company that has developed and patented a family of hyper stable, potent glycopeptides
(AAGP) that enhance both engraftment and protection of transplanted cells, organs, tissues and organs used in regenerative medicine.
sensitivity pattern in staphylococciisolated from clinical specimens in a tertiary care hospital.
, this condensation occurs in side chain of amino acid but introduction of isonitrile moiety on N-terminal of amino acid mimic unit can also be a route to produce such compounds.
The vanA gene contributes higher resistance to vancomycin and teicoplanin both glycopeptides
while VanB gene which is also an effective resistant gene encodes resistance to vancomycin only.
Over the past decades, Methicillin resistance in CoNS has substantially increased and has led to the use of Glycopeptides
like Vancomycin and Teicoplanin.
The next step we're taking is to identify all those differential glycopeptides
, to break it down to single biomarkers, which helps us to elucidate the mechanism of disease and develop novel personalized diagnostics."
LC-ESI-MS/MS is a robust, high-throughput method in identifying and quantifying glycopeptides
in enzymatic digests from various proteomic samples.
Multiple phenotypes with decreased sensitivity to glycopeptides
are on the rise due to increased vancomycin usage in critically ill patients with MRSA infections.15 It is possible that vancomycin treatment failures against putatively vancomycin-susceptible Staphylococcus aureus are due to undiagnosed VISA or hVISA.16
Inducible genes encoding these phenotypes alter cell wall synthesis and render strains resistant to glycopeptides
.  Van A and Van B types of resistance are primarily found among Enterococci isolated from clinical, veterinary, and food specimens but no other common intestinal or environmental bacteria.
(4),(5) S-glycosylation is rare, and only observed in glycopeptides
. 6) Due to its nature, the biosynthesis of glycoproteins can give rise to the formation of a plethora of compounds that have in common identical protein chains, but differ in structure of the glycan chains and/or in occupancy of the glycosylation sites.
We applied our previously reported affinity-chromatography method in combination with a bottom-up proteomics approach comprising an enzymatic deglycosylation scheme to retain a small glycan moiety attached to the glycopeptides
as direct proof of glycosylation, single as well as combined enzymatic protein digestion, [beta]-elimination with concomitant Michael addition chemistry, and high-resolution nano-LC-MS (3, 23, 24).