Comparison of glyceraldehyde-3-phosphate
dehydrogenase and 28s-ribosomal RNA gene expresslon in human hepatocellular carcinoma.
Caption: Figure 4: (a and b) Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of glutathione-S transferase peroxidase micro ribonucleic acid (RNA) expressions and corresponding glyceraldehyde-3-phosphate
dehydrogenase in mammary gland tumor.
In brief, quantitative real-time PCR was performed in triplicate in 96-well plates containing SYBR Green PCR Master Mix and 10 pM forward primer and reverse primer for CD molecules and glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) has been considered a classical cytosolic glycolytic protein and in some microorganisms plays some role in cell flocculation (8).
5) Human genes: TBP, TATA box binding protein; UBC, ubiquitin C; SDHA, succinate dehydrogenase complex, subunit A, flavoprotein (Fp); RLP13, receptor like protein 13; YHWAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; HMBS, hydroxymethylbilane synthase; B2M, fi-2 microglobin; hypoxanthine phosphoribosyltransferase 1; GAPDH, glyceraldehyde-3-phosphate
dehydrogenase; RPS18, ribosomal protein S18; ACTB, actin, beta; GSS, glutathione synthetase (hGus); 36B4, acidic ribosomal phosphoprotein P0.
dehydrogenase (GAPDH) housekeeping gene was used for normalization of EPCR gene expression data.
A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate
Glucose is converted to glyceraldehyde-3-phosphate
and dihydroxyaceotone phosphate.
We then used semiquantitative RT-PCR with glyceraldehyde-3-phosphate
dehydrogenase as an internal reference to detect the virus and determine the amount of the viral RNA in the medium.
Using a housekeeping gene, glyceraldehyde-3-phosphate
dehydrogenase (GAPDH), as an internal control, the mRNA expression of different molecular markers was analyzed by semiquantitative RT-PCR as described previously.
The glycolytic enzyme, glyceraldehyde-3-phosphate
dehydrogenase, was down-regulated (Lau et al.
Christopher Keller, Minot State University 9:30 Welcome and Overview 9:40 "Brownian dynamics of rabbit glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) mutants binding rabbit F-actin.